外源性一氧化碳释放分子2对大鼠脊髓缺血再灌注损伤的保护作用研究  

Protective effects of exogenous carbon monoxide releasing molecules 2in rat with spinal cord ischemia and reperfusion injury

作　　者：刘志刚[1] 熊敏[1] 余化龙[1] 曾云[1] 

机构地区：[1]湖北医药学院附属东风医院骨科研究所,湖北十堰442000

出　　处：《检验医学与临床》2015年第8期1048-1050,共3页Laboratory Medicine and Clinic

基　　金：湖北省十堰市科技局基金支持项目[十科发(2013)20号]

摘　　要：目的探讨外源性一氧化碳释放分子2(CORM2)对大鼠脊髓缺血再灌注损伤的保护作用与相关机制。方法将30只健康雄性Wistar大鼠分为实验组、对照组、空白组,每组各10只。实验组在造模前1h尾静脉注射外源性CORM2。实验组和对照组采用相同的方法造模。对照组造模后1h注射与实验组相同剂量的生理盐水。空白组仅完成开腹和关腹操作,并术后1h注射与实验组相同剂量的生理盐水。在术后不同时间进行神经行为学评分(Tarlov评分)、脊髓组织病理学检查和白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)检测。结果再灌注后不同时间点,实验组Tarlov评分均高于对照组,但实验组和对照组Tarlov评分均低于空白组(P<0.05)。缺血再灌注48h后,对照组脊髓组织标本可见损伤及坏死神经元,表现为细胞核固缩或溶解,细胞质中的尼氏体消失;空白组脊髓组织标本中可见正常神经元,表现为结构呈多角形,细胞质中可见尼氏体;实验组脊髓组织标本中的神经元坏死表现介于对照组和空白组之间。缺血再灌注48h,实验组与对照组IL-6、TNF-α表达水平均高于空白组,但实验组IL-6、TNF-α表达水平低于对照组(P<0.05)。结论外源性CORM2可抑制炎性因子的表达,对大鼠脊髓缺血再灌注损伤具有一定的保护作用。Objective To investigate the protective effects of exogenous carbon monoxide releasing molecules 2（CROm^2）in rat with spinal cord ischemia and reperfusion injury.Methods A total of 30 healthy male Wistar rats were divided into experiment group,control group and blank group,with 10 rats in each group.Rats of experiment group were given exogenous CORm^2 by intravenous injection 1hbefore modeling.Same operation was performed in experiment group and control group to construct rat models.Rats in control group were injected the same dose of saline 1hafter the construction of models.Rat in blank group were given only abdominal operation without any other treatment,and were injected the same dose of saline 1hafter operation.At different time point of operation,evaluation of Tarlov scale,histopathology examination of myeloid tissue,and detection of interleukin-6（IL-6）and tumor necrosis factor-α（TNF-α）were performed.Results At different time point of reperfusion,Tarlov scores of experiment group were higher than control group,but those of the two groups were lower than blank group（P〈0.05）.At48 hafter ischemia-reperfusion,damage of neuron in control group was obvious,which was not found in blank group,and the characteristics of neuron damage in experiment group were between control group and blank group.At 48 h after ischemia-reperfusion,levels of IL-6and TNF-αin experiment group and control group were significantly higher than blank group,while those in experiment group were significantly lower than control group（P〈0.05）.Conclusion Exogenous CORm^2 could be with protective effects of spinal cord ischemia-reperfusion injury,which might be dependent on the inhibition of the releasing of inflammatory cytokines.

关 键 词：外源性一氧化碳释放分子2 脊髓缺血再灌注损伤 白细胞介素-6 肿瘤坏死因子-Α 

分 类 号：R971.2[医药卫生—药品]