抗心律失常肽对抗柯萨奇B3病毒的体外研究  

The vitro study of anti-arrhythmic peptide against coxsackie B3 virus

作　　者：伍琼[1,2,3] 盛蕾[1,2,3] 洪李锋[1,2,3] 

机构地区：[1]湖北省武汉市第五医院心血管内科 [2]武汉大学广慈医院心血管内科 [3]江汉大学心血管研究所,430050

出　　处：《检验医学与临床》2015年第8期1056-1058,共3页Laboratory Medicine and Clinic

基　　金：湖北省武汉市汉阳区科学技术局计划项目[阳科(2011)11号]

摘　　要：目的观察抗心律失常肽(AAP10)对柯萨奇B3病毒(CVB3)感染的原代小鼠心肌细胞的保护作用。方法将BALB/C小鼠原代心肌细胞分为7组,每组12孔,分别为细胞对照组,病毒对照组,药物干预组(A组、B组、C组、D组、E组)。用不同浓度的AAP10培养液干预100TCID50CVB3感染的心肌细胞,对比每组的细胞活性。结果药物干预组细胞活性高于病毒对照组(F=7.489,P<0.05)。其细胞活性与药物浓度(r=0.942,P=0.005)及作用时间(r=0.993,P<0.05)均呈正相关。结论 AAP10具有明显的抗病毒活性,呈剂量时间相关性,对CVB3感染的心肌细胞有保护作用。Objective To observe the anti-arrhythmic peptide 10（AAP10）against coxsackie virus B3（CVB3）damage to the generation of myocardial cells in mice.Methods BALB/C mice primary myocardial cells can be divided into 7groups,each group of 12holes：the cell control group,the virus control group,and drug intervention group（five different concentrations）.Myocardial cells of 100TCID50CVB3 were interventied with different concentrations of AAP10.Cell damage degree among each group were contrasted.Results Myocardial activity has a positive correlation with the drug concentration（r=0.942,P=0.005,r^2=0.888）and action time（r=0.993,P〈0.05）.Myocardial activity in intervention group is significantly higher than virus control group（F=7.489,P〈0.05）.ConclusionAAP10 has obvious antiviral activity in vitro,which has a correlation with drug and action time.AAP10 can protect myocardial cell infected by CVB3 in vitro.

关 键 词：抗心律失常肽 柯萨奇B3病毒 原代心肌细胞 

分 类 号：R373.23[医药卫生—病原生物学]