人尿激肽原酶对脑缺血再灌注模型大鼠脑源性神经营养因子的影响  

Effect of urinary kallidinogenase on the expression of brain derived neurotrophic factor in rats with focal cerebral ischemia-reperfusion

作　　者：李国前[1] 王杰华[1] 杨小霞[1] 洪诸权[1] 留晓强[1] 

机构地区：[1]福建医科大学附属泉州第一医院神经内科,福建泉州362000

出　　处：《中国临床药理学杂志》2015年第8期624-626,共3页The Chinese Journal of Clinical Pharmacology

基　　金：泉州市科技计划基金资助项目(2013Z59)

摘　　要：目的观察人尿激肽原酶对脑缺血再灌注损伤模型大鼠脑组织中脑源性神经营养因子表达的影响。方法将SD大鼠随机分为假手术组、模型组和实验组,每组8只。实验组于再灌注后5 min静脉注射人尿激肽原酶3.5×10-3PNA U·kg-1;假手术组和模型组正常喂养。模型组和实验组用线栓法制作大脑中动脉脑缺血再灌注模型,假手术组大鼠接受相似手术处理,但是不插入线栓。在缺血2 h再灌注48 h后,断头取脑,用半定量聚合酶链式反应法、免疫组织化学法和Western blot法检测脑组织中脑源性神经营养因子的表达水平。结果与假手术组相比,模型组脑组织中脑源性神经营养因子的表达明显增高(P<0.05);与模型组相比,实验组脑组织中脑源性神经营养因子的表达明显增高(P<0.05)。结论人尿激肽原酶可增加脑缺血再灌注损伤后脑组织中脑源性神经营养因子的表达,对脑缺血再灌注损伤起保护作用。Objective To observe the effects of urinary kallidinogenase on the expression of brain derived neurotrophic factor（BDNF） in rats with focal cerebral ischemia-reperfusion.Methods SD rats were randomly assigned into three groups： sham operation group（sham group）,focal cerebral ischemia-reperfusion group（model group） and urinary kallidinogenase group（test group）,with eight in each group.The middle cerebral artery occlusion reperfusion model was made by the suture method（ischemia for 2 hours,and reperfusion for 48 hours）.The rats in the test group were injected with urinary kallidinogenase 3.5 × 10^-3PNA U·kg^-1after making a model.The level of BDNF expression were measured by PCR,immunohistochemistry and Western blot.Results Compared with sham group,the expression of BDNF was increased in model group（P〈0.05）.Compared with model group,the expression of BDNF was increased in test group（P〈0.05）.Conclusion Urinary kallidinogenase has a protective effect on ischemic brain injury by increasing the BDNF expression in ischemic brain.

关 键 词：人尿激肽原酶 线栓法 大鼠 脑缺血再灌注 脑源性神经营养因子 

分 类 号：R977.3[医药卫生—药品] R965.1[医药卫生—药学]