康脑液预处理对局灶性脑缺血再灌注大鼠海马CA1区神经再生的影响  

Effects of Kangnaoye recipe preconditioning on nerve regeneration in rats CA1 area after cerebral ischemia and reperfusion

作　　者：常青[1] 马建鹏[1] 邹玉安[2] 薛茜[2] 

机构地区：[1]河北北方学院研究生院,河北张家口075000 [2]河北北方学院附属第一医院神经内科,河北张家口075000

出　　处：《中国临床药理学杂志》2015年第8期631-634,共4页The Chinese Journal of Clinical Pharmacology

基　　金：河北省卫生厅课题基金资助项目(20090591)

摘　　要：目的观察康脑液预处理后对大鼠局灶性脑缺血再灌注脑组织CA1区神经再生的影响。方法将150只健康雄性Sprague-Dawley大鼠随机分为康脑液高、中、低3个剂量组(24,12,6 g·kg-1·d-1)、假手术组及模型组,建立右侧局灶性脑缺血模型,于缺血后2 h及24 h进行神经功能缺损评分。于再灌注1,3,7,14 d后,通过HE染色观察海马CA1区细胞的形态学改变,免疫组化检测各组CA1区胶质纤维酸性蛋白(GFAP)和巢蛋白(Nestin)的表达。结果康脑液预处理组在术后的神经功能缺损评分明显降低,能显著抑制CA1区GFAP的表达(P<0.01),并显著增强CA1区Nestin的表达(P<0.01)。结论康脑液抗脑缺血再灌注损伤可能与促进海马区神经再生及分化、抑制纤维瘢痕形成有关。Objective To explore the influence of Kangnaoye recipe preconditioning on the nerve regeneration in rats CA1 area after cerebral ischemia and reperfusion.Methods The 150 rats were randomly divided into sham-operated group,model group,high-dose Kangnaoye group,middle-dose Kangnaoye group and low-dose Kangnaoye group（24,12,6 g·kg^-1·d^-1）.The modle of cerebral ischemia and reperfusion（I / R） was induced by middle cerebral artery occlusion（MCAO）and recanalization.Neurological symptom score were evaluated.HE staining of the cells was used to observe the morphology changes in hippocampus CA1 area.Glial ibrillary acidic protein（GFAP） protein and Nestin protein were observed by immunohistochemical method in CA1 area.Results Compared with model group,neurological symptom score of Kangnaoye recipe preconditioning group were decreased and the result was significant（P〈0.01）.The groups pretreated with Kangnaoye had a lower expression of GFAP than the model group（P〈0.01） and the expression of the Nestin of groups pretreated with Kangnaoye were significantly higher than the model group（P〈0.01） at the time of the 1,3,7and 14 d after cerebral ischemia.Conclusion The Kangnaoye recipe preconditioning had protective effects on the pathological morphological changing,it may be associated with promoting the neural stem cells regeneration and differentiation as well as inhibition the formation of fibrous scar.

关 键 词：脑缺血再灌注 康脑液 胶质纤维酸性蛋白 神经再生 

分 类 号：R285.5[医药卫生—中药学] R977.9[医药卫生—中医学]