藏药独一味中的主要化学成分环烯醚萜苷化合物在动物的镇痛抗炎作用研究  被引量:29

Analgesic and anti-inflammatory activities of iridoid glycosides from Tibetan medicine Lamiophlomis rotata in Balb / C mice

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作  者:贾诩 李茂星[2,3] 陶锐[3] 傅仲学[1] 

机构地区:[1]重庆医科大学附属第一医院胃肠外科,重庆400016 [2]兰州军区兰州总医院药剂科全军高原环境损伤防治重点实验室,兰州730050 [3]兰州大学药学院,兰州730000

出  处:《中国临床药理学杂志》2015年第8期635-639,共5页The Chinese Journal of Clinical Pharmacology

基  金:国家科技部重大新药创制基金资助项目(2008ZXJ09004-52);甘肃省科技重大专项基金资助项目(1102FKDA012);甘肃省中医药管理局科研课题基金资助项目(GZK-2014-1)

摘  要:目的比较独一味中环烯醚萜苷化合物在小鼠体内的镇痛与抗炎活性。方法 5种环烯醚萜苷化合物:8-乙酰氧基山栀子苷甲酯(Ⅰ)、8-去羟基山栀子苷(Ⅱ)、sesamoside(Ⅲ)、7,8-dehydro-penstemoside(Ⅳ)、山栀子苷甲酯(Ⅴ),均在小鼠尾静脉一次注射给药200 mg·kg-1,阳性对照组给予吗啡5 mg·kg-1,空白对照组给予0.9%氯化钠。用经典的动物实验模型,评价其体内镇痛、抗炎作用。结果给药后5种单体化合物均显示了一定的镇痛抗炎活性,其中Ⅰ和Ⅱ都能显著减少小鼠扭体次数,抑制二甲苯引起的小鼠耳肿胀,抑制足部注射福尔马林所致第二阶段疼痛反应,抑制角叉菜胶诱导小鼠足肿胀,降低醋酸诱导的腹腔通透性的增加,减少腹腔内伊文思蓝含量和腹腔白细胞游走。但不能延长小鼠热板痛阈值,不能减少福尔马林致疼痛第一阶段舔足次数。结论藏药独一味中的5种环烯醚萜苷单体,尤其是8-乙酰氧基山栀子苷甲酯和8-去羟基山栀子苷具有较好的体内镇痛、抗炎作用。Objective To study the analgesic and anti-inflammatory activities of iridoid glycoside fromLamiophlomis rotata in mice.Methods After one dose of iridoid glycosides(200 mg · kg^-1),8-O-acetylshanzhiside methylester,8-deoxyshanzhiside,sesamoside,7,8-dehydropenstemoside,and shanzhiside methylester,were administered by tail vein injection.Morphine(5 mg · kg^-1) and aspirin(100mg·kg^-1) were used as positive controls,and 0.9% NaCl as negative control.The hot plate test,acetic-acid induced writhing test,formalin test,carrageenan-induced paw edema test and xylene-induced ear swelling test,peritoneal capillary permeability test on mice were used to test the analgesic and anti-inflammatory activities.Results Compared with blank group,these five iridoid glycosides could remarkably induce the number of writhing reaction with the inhibition ratio were 43.96%,47.81%,25.96%,37.70%,and 26.23%(P〈0.01,P〈0.01,P〈0.05,P〈0.01,and P〈0.05); could inhibit the xylene-induced ear swelling were 0.79%(P〈0.05),43.07%(P〈0.01),7.90%,14.25%,and 5.59%; exhibited significant analgesic activities on the second period of formalin test by 8-O-acetylshanzhiside methylester and 8-deoxyshanzhiside(P〈0.01).All compounds exhibited effectson paw edema induced by carrageenan in 1 hour,but only 8-O-acetylshanzhiside methylester and 8-deoxy-shanzhiside were effective in 3 hours with inhibition ratio were 32.84% and 40.36%,respectively.They also reduced permeability of abdominal capillaries and inflammatory peritoneal exudation induced by acetic acid,especial for 8-O-acetylshanzhiside methylester,8-deoxyshanzhiside and sesamoside.On the other hand,these iridoid glycosides couldn't prolong the leg-licking time in hot plate test and the first period of formalin test.Conclusion The five iridoid glycosides,espicially 8-O-acetylshanzhiside methylester and 8-deoxyshanzhiside exhibited significant analgesic and anti-inflammatory activities.

关 键 词:环烯醚萜苷 8-去羟基山栀子苷 镇痛 抗炎 

分 类 号:R285.5[医药卫生—中药学] R971.1[医药卫生—中医学]

 

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