β_2 GP1诱导的EAPS小鼠Treg/Th17细胞比率变化的研究  

Changes of Treg / Th17 in mice of β_2 glycoprotein 1 induced experimental antiphospholipid syndrome

作　　者：徐立[1] 王峻松[1] 闫岩[2] 刘磊[3] 戴鹤玲[4] 付嘉[5] 方艳秋[3] 谭岩[3] 

机构地区：[1]吉林大学第二医院检验科,长春130041 [2]吉林省抚松县人民医院检验科,抚松134500 [3]吉林省人民医院中心实验室,长春130021 [4]北京军区总医院骨科,北京100700 [5]济宁医学院基础学院免疫教研室,济宁272067

出　　处：《中国免疫学杂志》2015年第4期467-471,共5页Chinese Journal of Immunology

基　　金：吉林省科技厅自然基金项目(No.201015172);吉林省卫生厅项目(No.20112074;No.2010Z101-263)

摘　　要：目的:观察EAPS疾病进展过程中小鼠外周血Th17细胞与CD4+CD25+调节性T(Treg)细胞的比率变化。方法:以重组人β2糖蛋白1(rhβ2GP1)主动免疫BALB/c小鼠建立EAPS模型,免疫12周后检测外周血浆抗β2糖蛋白1抗体(anti-β2GP1)、抗心磷脂抗体(a CA)、IL-17、IL-2、IL-6、TGF-β、活化部分凝血活酶时间(APTT)和血小板计数(PLT)及流产率。流式细胞术(FCM)检测小鼠PBMC中CD4+CD25+Treg及Th17细胞的比率。结果:与对照组相比,模型小鼠anti-β2GP1、a CA、IL-17、IL-2、IL-6水平明显升高,APTT延长,TGF-β降低,PLT升高,流产率提高,差异有统计学意义(P<0.05)。PBMC中CD4+CD25+Treg细胞频率8周前与对照组相比差异无统计学意义(P>0.05),12周后逐渐减少,明显低于对照组(P<0.05);Th17细胞频率逐渐升高,明显高于对照组(P<0.05);CD4+CD25+Treg/Th17比值明显低于对照组(P<0.05)。结论:EAPS小鼠外周血Th17/Treg细胞比率失衡可能在EAPS的发生发展中起重要作用。Objective：To observe the change of CD4＋CD25＋regulatory T cells and Th17 cells in mice with experimental antiphospholipid antibody syndrome（ EAPS）. Methods： EAPS model was established by immunizing BALB / c mice with recombinant human β2glycoprotein 1（ rhβ2GP1）. The levels of serum anti-β2glycoprotein 1（ anti-β2GP1）,anti-cardiolipin antibody（ a CA）,IL-17,IL-2,IL-6 and TGF-β were tested by ELISA. The rate of abortion,activated partial thromboplastin time（ APTT） and platelet count were also detected. Flow cytometry was applied to detect the percentages of the CD4＋CD25＋regulatory T cells and Th17 cells in peripheral blood mononuclear cells. Results： Compared with the control group,the levels of anti-β2GP1,a CA,IL-17,IL-2 and IL-6 were significantly increased,the rate of abortion was increased,APTT time was prolonged and the levels of TGF-β and platelet count were decreased in model mice（ P〈0. 05）. No significant difference was detected of percentage of Treg cells in PBMC at the eighth weeks in model group（ P〉0. 05）,but percentage of Treg cells was lower than that in control group after 12 weeks（ P〈0. 05）;the percentage of Th17 cells in model group was higher than that in control group（ P〈0. 05）. In addition,the ratio of Treg / Th17 cells was lower in model mice than that in control group. Conclusion： The imbalance of CD4＋CD25 Treg / Th17 cells may participate in the pathogenesis of EAPS.

关 键 词：抗磷脂抗体综合征 调节性T细胞 TH17细胞 

分 类 号：R392[医药卫生—免疫学]