过氧化氢诱导不同色素水平葡萄愈伤组织白藜芦醇及白藜芦醇苷含量差异  

The Differences Between Resveratrol and Piceid in Grapevine Callus Responsed to the Hydrogen Peroxide Treatment

作　　者：李月荣[1] 颜欢[1] 张波[1,2] 杨晓燕 李荣飞[1] 王晓琴[1] 

机构地区：[1]石河子大学药学院,新疆石河子832002 [2]省部共建新疆特种资源植物药重点实验室,新疆石河子832002

出　　处：《北方园艺》2015年第8期114-116,共3页Northern Horticulture

基　　金：国家自然科学基金资助项目(31160058);新疆兵团重点领域科技攻关资助项目(2014BA029)

摘　　要：以不同状态的"红地球"葡萄愈伤组织为试材,采用高效液相色谱法,研究H2O2对其白藜芦醇和白藜芦醇苷含量的影响。结果表明:经H2O2诱导后3种愈伤组织中白藜芦醇苷含量为:红色愈伤组织>褐化的愈伤组织>白色愈伤组织,而白藜芦醇在白色愈伤组织中的含量高于红色和褐化的愈伤组织。2.5μM H2O2诱导红色愈伤组织24h后,白藜芦醇苷含量高达306μg/gFW,而白藜芦醇的含量变化不明显。红色愈伤组织中的白藜芦醇苷响应H2O2的胁迫较为明显,其对H2O2最佳的响应浓度为2.5μM。Taking three different stages grape callus（Vitis vinifera I.. cv. Red Globe） as test materials,with H2 O2 solution to investigate the effect of resveratrol and piceid content by HPLC. The results showed that the content of piceid in red callus was the highest,following by brown callus,and white callus. The content of resveratrol in white callus was higher than red and browning callus. The maximum content of piceid 306 μg/g FW in treated red callus was achieved by 2. 5μM H2 O2 for 24 hours treatment. It was not significant tb.at the content of resveratrol changes in three different stages grape callus. It was very obvious that the response of piceid in red callus to H2O2 ,which limited to 2. 5 μM H2O2.

关 键 词：葡萄愈伤组织 白藜芦醇 白藜芦醇苷 过氧化氢 

分 类 号：S663.1[农业科学—果树学]