蔬菜根结线虫生防细菌的筛选及菌株HJT2-1种类的初步鉴定  

The Screening of Biocontrol Bacteria Against Vegetable Root-knot Nematode and Identification of Bacterial Strain HJT2-1

作　　者：霍建飞[1] 任文来[2] 郝永娟[1] 刘春艳[1] 王勇[1] 王万立[1] 

机构地区：[1]天津市植物保护研究所,天津300381 [2]廊坊市文安县农业局,河北廊坊065800

出　　处：《北方园艺》2015年第8期120-123,共4页Northern Horticulture

基　　金：天津市农科院院长基金资助项目(090401)

摘　　要：以天津地区7个区县的蔬菜大棚土壤中分离出的306株细菌菌株为试材,采用亚甲基蓝染色法,研究306株细菌菌株发酵液对南方根结线虫2龄幼虫的影响。结果表明:细菌菌株HJT2-1发酵液对南方根结线虫2龄幼虫的校正死亡率较高为81.3%,其温室盆栽防效为55.6%,略低于阿维菌素防效。采用对细菌核糖体DNA的内转录间隔区进行分析的方法将菌株HJT2-1初步鉴定为假单胞菌属细菌。该试验获得了对根结线虫2龄幼虫具有较高致死率的细菌菌株HJT2-1,为开发防治根结线虫的生防制剂奠定了基础。Taking 306 bacterial strains which were isolated from vegetable soils of greenhouses in seven different areas of Tianjin as material, the effect of fermentation liquor of 306 bacterial strains on the death rate of Meloidogyn incognita （J2） by using methylene blue stain method. The results showed that the bacterial strain HJT2-1 caused more than 81.3% of corrected mortality against J2, and the control effect in greenhouse could reach to 55. 6% and was lower than Avermectin. The bacterial strain HJT2-1 was preliminarily identified as Pseuclornonas by analyzing the sequence of bacterial ribosomal DNA internal transcription space. This experiment obtained strain HJT2-1 which was effective to J2, and laid a solid foundation to develop bioeontrol preparations against root-knot nematode.

关 键 词：蔬菜根结线虫 生防细菌 筛选 鉴定 

分 类 号：S436.44[农业科学—农业昆虫与害虫防治]