正交试验优选防感颗粒的提取工艺  

Optimum Extracting Procedure of Fanggan Granules by Orthogonal Design

作　　者：王京霞[1] 陈琳[1] 陈芳[1] 林燕飞 孙静芸 

机构地区：[1]浙江省中医院,杭州310006 [2]杭州创新中药标准化研究所有限公司,杭州310053

出　　处：《中国现代应用药学》2015年第4期428-432,共5页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的确立防感颗粒的最佳提取工艺。方法应用正交试验和HPLC,以防感颗粒中R,S-告依春、白花前胡甲素、苦杏仁苷及甘草苷4种活性成分含量为指标,采用多指标综合评分法,确定防感颗粒的提取工艺。结果防感颗粒最佳提取工艺为:药材加8倍量水,煎煮3次,每次1.5 h,乙醇浓度为60%。结论优选出的工艺稳定可靠,可用于防感颗粒的提取。OBJECTIVE To establish the optimum extracting procedure of Fanggan granules. METHODS A multi index comprehensive evaluation method by orthogonal test and HPLC was used for the determination of the extracting procedure of Fanggan granules, with four active ingredients including（R,S）-epigoitrin, praeruptorin A, amygdalin and liquiritin as the content index. RESULTS The optimum extraction process of Fanggan granules was determined as follows： herbs were extracted 3 times with 8 times of water; 1.5 h each time; ethanol concentration was 60%. CONCLUSION The optimum procedure is stable and reliable for the extraction of Fanggan granules.

关 键 词：防感颗粒 R S-告依春 苦杏仁苷 白花前胡甲素 甘草苷 正交试验 提取工艺 

分 类 号：R283.6[医药卫生—中药学]