市售枳壳饮片的质量调查与指纹图谱研究  

Study on the Quality Investigation and Fingerprint of Aurantii Fructus

作　　者：范蕾 吴查青 王伟影 陈卫平 毛菊华 陈张金 李水福 

机构地区：[1]丽水市食品药品检验所,浙江丽水323000

出　　处：《中国现代应用药学》2015年第4期432-436,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省公共科技条件平台首期建设项目(2012E60008);丽水市重点科技创新团队建设项目(2012CXTD11)

摘　　要：目的建立HPLC指纹图谱,调查16批市售枳壳饮片的质量。方法色谱柱为Agilent-SB C18(250 mm×4.6 mm,5μm),流动相为乙腈-水(20︰80)(用磷酸调节p H值至3),流速为1.0 m L·min?1,柱温30℃,检测波长为283 nm。利用相似度评价系统进行指纹图谱相似度计算。结果 16批市售枳壳饮片均达到药典标准,不同炮制方法所得枳壳含量略有差异。枳壳指纹图谱相似度>0.98。结论市售枳壳饮片质量较好。指纹图谱方法稳定、重复性好,可用于枳壳的质量控制。OBJECTIVE To investigate the quality of the 16 batches of commercial Aurantii Fructus by establishment of HPLC fingerprinting. METHODS HPLC was applied with the chromatographic condition as follows： the chromatographic column was Agilent Zorbax SB-C18（250 mm×4.6 mm, 5 μm） at 30 ℃; mobile phase was acetonitrile-water（20∶80）（adjusted to p H 3 with phosphoric acid）; flow rate was 1.0 m L·min-1, the detection wavelength was 283 nm. Used the similarity evaluation system for fingerprint similarity calculation. RESULTS Sixteen batches of commercial Aurantii Fructus were reaching pharmacopoeia standards, there were a little differences between two processing methods. Fingerprint similarity was above 0.98. CONCLUSION The method of fingerprint is stable, repeatable and it is suitable for the quality control of Aurantii Fructus.

关 键 词：枳壳 质量 指纹图谱 

分 类 号：R917.101[医药卫生—药物分析学]