UPLC测定钩吻素子的含量及其水溶液稳定性  

Content Determination and Stability of Koumine by UPLC

作　　者：叶丽香[1] 苏燕评[2] 曹大炫 林菁[1,2] 俞昌喜[1,2] 

机构地区：[1]福建医科大学福建省新药安全性评价中心,福州350108 [2]福建医科大学药学院,福州350108

出　　处：《中国现代应用药学》2015年第4期471-474,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：福建省自然科学基金资助项目(2013J01374);福建医科大学重大科研项目计划(09ZD009)

摘　　要：目的 建立超高效液相色谱法测定钩吻素子的含量,并考察其水溶液在室温条件下的稳定性。方法 采用AgilentExtend-C18（2.1 mm×100 mm,1.8μm）色谱柱,以乙腈-0.2%正丁胺水溶液（30∶70）为流动相,流速0.5 mL.min 1,检测波长256 nm,测定3个批次钩吻素子的含量,并考察钩吻素子水溶液在室温条件下稳定性。结果 钩吻素子分离度良好;在10.19~112.2μg.mL 1内线性关系良好（r=0.999 7）,定量限为0.1μg.mL 1;平均回收率为100.4%（RSD=0.45%,n=9）;3个批次钩吻素子平均含量为99.97%;钩吻素子水溶液室温条件下连续7 d稳定性良好。结论 该方法简单易行、专属性强,准确可靠,适用于钩吻素子含量测定,且本品水溶液稳定性良好。OBJECTIVE To establish a ultra high performance liquid chromatography（UPLC） method for determination of koumine and to study the stability of koumine solution at room temperature. METHODS The analysis was achieved by Agilent Extend-C18 chromatogram column（2.1 mm×100 mm, 1.8 μm）. The mobile phase composed of acetonitrile-0.2% butylamine（30∶70） at flow rate of 0.5 m L·min？1. The detection wavelength was set at 256 nm. Three batches of koumine were determined and stability of its solution was examined under room temperature. RESULTS Under the estabilished method, the separation of koumine and its main related substances was good. The linear range of koumine was 10.19？112.2 μg·m L？1（r=0.999 7） with detection limit of 0.1 μg·m L？1. The average recovery was 100.4%（RSD=0.45%, n=9）. The average concentration of koumine were 99.97%（n=3）. The koumine solution had excellent stability at room temperature in 7 d. CONCLUSION The UPLC method is a simple, rapid, accurate method for determination of koumine. The koumine solution has excellent stability at room temperature.

关 键 词：钩吻素子 超高效液相色谱法 含量测定 稳定性 

分 类 号：R917.101[医药卫生—药物分析学]