体液添加EDTA-K_2抗凝剂对细胞计数的影响  

Study on impact of EDTA-K_2 anticoagulant added into body fluid on cell count

作　　者：马科[1,2] 左路广 常晓彤[2] 连晶晶[1] 冯博[1] 

机构地区：[1]河北北方学院附属第一医院,河北张家口075000 [2]河北北方学院,河北张家口075000

出　　处：《医疗卫生装备》2015年第4期91-93,共3页Chinese Medical Equipment Journal

基　　金：河北省高等学校科学技术研究青年基金项目(Q2012141)

摘　　要：目的:研究添加EDTA-K2抗凝剂对体液细胞计数的影响。方法:由临床送检的110份体液中取1、2、4、2 m L依次加入3支EDTA-K2抗凝剂及1支不含抗凝剂试管中,选用Sysmex XE-5000全自动血细胞分析仪测定白细胞(WBC)、红细胞(RBC)、单个核细胞(MN)、多个核细胞(PMN),分析添加EDTA-K2抗凝剂对体液细胞计数的影响;另收集体液,送检后及时测量并分装EDTA-K2抗凝剂及无抗凝剂试管中各2 m L,4℃冷藏2、4、6、12 h分别测量细胞含量,分析体液采集后不同时间细胞数量的变化趋势。结果:体液添加EDTA-K2抗凝剂各组相对于对照组细胞计数均无统计学差异;体液抽取后如无抗凝剂,随放置时间延长,白细胞计数出现下降趋势,EDTA-K2抗凝体液则无下降趋势。结论:体液中添加EDTA-K2抗凝剂可提高体液细胞计数的准确性,减小临床体液标本不能及时检测对细胞计数的影响。Objective To evaluate the impact of adding EDTA-K2 anticoagulant to body fluid on humoral cell count.Methods There were 1, 2, 4 and 2 m L of body fluid were respectively extracted from each of 110 clinical specimens and then dropped into three tubes with EDTA-K2 anticoagulant and one empty tube respectively. Sysmex XE-5000 automatic blood cell analyzer was used to detect WBC, RBC, MN and PMN so as to analyze the impact of EDTA-K2 anticoagulant on humoral cell count. Some other specimens were tested, and then two 2 m L of body fluid were drawn from them and dropped into one tube with EDTA-K2 anticoagulant and one empty tube respectively, cell count was carried out after the tubes were in the condition of 4 ℃ for 2, 4, 6 and 12 hours to determine the trend that cell count changed with the time going by. Results EDTA-K2 anticoagulant had no significant impact on cell count, while WBC count decreased with the time going by when EDTA-K2 anticoagulant was absent. Conclusion Adding EDTA-K2 anticoagulant into the body fluid can enhance the accuracy of humoral cell count, and can relieve the influence of delayed detection on cell count.

关 键 词：体液 EDTA-K2抗凝剂 细胞计数 

分 类 号：R446.1[医药卫生—诊断学]