新疆圆柏总黄酮对大鼠佐剂性关节炎的防治作用  

Preventive and Therapeutical Effects of Total Flavonoids from Juniperus sabina on Adjuvant-induced Arthritis in Rats

作　　者：刘涛[1] 许芳[1] 赵军 由淑萍[1] 徐芳 

机构地区：[1]新疆医科大学公共卫生学院,乌鲁木齐830011 [2]新疆药物研究所-维吾尔药重点实验室,乌鲁木齐830004

出　　处：《天然产物研究与开发》2015年第4期710-714,共5页Natural Product Research and Development

基　　金：国家自然科学基金(81160515)

摘　　要：利用弗氏完全佐剂(FCA)诱导的佐剂性关节炎(AA)模型对新疆圆柏总黄酮(JSTF)的抗炎作用进行研究。致炎前预防性给予JSTF后,AA大鼠急性期足肿胀的趋势有一定的改善,其血清IL-1β和TNF-α水平也显著降低(P<0.05)。炎症继发期预防给药后,JSTF不同剂量组均可不同程度抑制大鼠皮肤的红斑、溃烂和降低外周血中TNF-α水平(P<0.05),且高剂量组能明显降低关节炎指数(AI)(P<0.05),并呈剂量-效应关系。炎症继发期治疗给药后,各给药组大鼠皮肤出现皱褶,皮肤色泽由暗红色变为正常,肿胀消退,继发侧的肿胀和红斑也有明显改善;与模型组比较,各组大鼠的AI值有明显的下降(P<0.05),其中高、低剂量组还能显著降低TNF-α水平(P<0.05)。结果表明JSTF对大鼠佐剂性关节炎具有一定的预防和治疗作用。To investigate the preventive and therapeutical effects of total flavonoids from Juniperus sabina （JSTF） on Freund＇ s complete adjuvant （FCA）-induced arthritis in rats. JSTF was administered intragastricaUy once a day before adjuvant-induced arthritis （AA） induction,JSTF can reduce rat right paw （injected side） swelling, and significantly im- prove IL-1 β and TNF-α level （P 〈 0.05 ）. In preventive experiment of secondary inflammatory, JSTF （ 125,250,500 mg/kg） can inhibit the degrees of skin erythema in rats ,and reduce the TNF-α levels in peripheral blood （P 〈0.05）, of which high-dose group significantly reduce arthritis index （AI） （ P 〈 0.05 ） at a dose-respense manner. In therapeutical experiment of secondary inflammatory, skin appeared folds with color from dark red to normal, secondary swelling and erythema significantly improved, compared with the model group, AI values of various group in JSTF were significantly decreased （P 〈 0.05）, of which the high and low dose group also significantly reduced TNF-α levels （P 〈 0.05 ）. The results showed that JSTF had preventive and therapeutical effects on adjuvant-induced arthritis in rats.

关 键 词：新疆圆柏 黄酮 佐剂性关节炎 

分 类 号：R285.5[医药卫生—中药学]