植物多糖抗氧化体外实验方法研究进展  

Research Progress on Experimental Methods on Testing in Vitro Antioxidant Activity of Plant Polysaccharides

作　　者：张德华[1] 邓辉[1] 乔德亮[1] 

机构地区：[1]皖西学院生物与制药工程学院,六安237012

出　　处：《天然产物研究与开发》2015年第4期747-751,共5页Natural Product Research and Development

基　　金：国家自然科学基金(31271853)

摘　　要：生物活性多糖具有多种生理活性和特殊保健功能,其抗氧化能力强弱与多糖的来源、组成性质和结构有关。随着多糖市场需求增加,快速、灵敏和实用的抗氧化检测方法尤为重要。目前检测主要在体外化学模拟、细胞水平和个体水平等三个平台上进行。本文简要介绍近年来植物多糖抗氧化体外化学模拟实验的研究方法和原理:包括多糖清除·OH的测定、清除DPPH·的测定、清除O-·2的测定、抗氧化活性的测定、还原力的测定、清除亚硝酸盐的测定和清除R·的测定,并评价各种方法的优缺点和应用范围。Biologically active polysaccharide has many kinds of physiological activities and health care functions. The strength of antloxidant capacity of plant polysaccharides is related with its sources, properties and structures. With the increasing demand of polysaccharide market, some test methods of antioxidant activities which are quick, sensitive and practical are particularly important. At present the tests are mainly used in the following three platforms ：in vitro chemical simulation, cellular level and individual level. This paper briefly reviews the methods and principles of detecting antioxidant activities of plant polysaccharides by in vitro chemical simulation experiment,including polysaccharides scavenging free radicals of hydroxyl,2,2-diphenyl-l-picryl-hydrazyl, super-oxlde anion and alkyl, reducing power, antioxldant activity and scavenging nitrites. The paper also evaluates the advantages and disadvantages of various test methods and their applications.

关 键 词：植物多糖 抗氧化 自由基 清除能力 

分 类 号：Q-33[生物学]