亚贡叶提取物中总酚酸的含量测定方法研究  

Study on Content Determination of Total Phenolic Acids in Extract of Yacon Leaves

作　　者：魏旭[1] 冉小库[1] 李德伟[1] 窦德强[1] 董锋[2] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]珍奥集团股份有限公司,辽宁大连116600

出　　处：《辽宁中医杂志》2015年第4期811-814,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：辽宁省高等学校创新团队课题(LT2013020);辽宁省教育厅一般项目(L2013364)

摘　　要：目的：建立亚贡叶提取物中总酚酸的含量测定方法。方法：以0.6%三氯化铁-0.9%铁氰化钾混合溶液作为显色剂,采用比色法测定亚贡叶提取物中总酚酸的含量。结果：亚贡叶提取物中总酚酸分析的线性范围为3~12μg/m L,回归方程为Y=68.410X-0.0087（r=0.9999）,平均回收率为98.95%,RSD=2.3%。结论：本方法操作简便、重现性好,可用于亚贡叶提取物中总酚酸的含量测定。Objective：To establish the content determination method of total phenolic acids in the extract of yacon leaves.Methods：The colour-developing agent was the mixture solution of 0.6% Fe Cl3-0.9% K3[Fe（CN） 6].The content of total phenolic acids in the extract of yacon leaves was determined by UV-spectrophotometry.Results：Calibration graphs were constructed in the range of 3 ~ 12 μg / m L for total phenolic acids.The average recovery of total phenolic acids was 98.95% with RSD = 2.3%.Conclusion：The method is simple,accurate and reliable for the content determination of total phenolic acids in the extract of yacon leaves.

关 键 词：亚贡 总酚酸 含量测定 

分 类 号：R927.2[医药卫生—药学]