肺炎支原体感染鼠T淋巴细胞活化的动态变化  

Dynamic Changes of T-Lymphocyte Activation in Mycoplasma pneumoniae Infected Rats

作　　者：赵芝娜[1,2] 刘宝山[3] 冯辉[4] 杨颖[1] 司文[1] 王桂珍[1] 

机构地区：[1]中国医科大学基础医学院病原生物学教研室,辽宁沈阳110001 [2]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016 [3]沈阳农业大学畜牧兽医学院,辽宁沈阳110866 [4]中国医科大学基础医学院免疫学教研室,辽宁沈阳110001

出　　处：《微生物学杂志》2015年第1期49-53,共5页Journal of Microbiology

基　　金：辽宁省社会科学发展基金项目(辽科发2012-46)

摘　　要：动态观察肺炎支原体(Mycoplasma pneumoniae,Mp)感染鼠T淋巴细胞的数量及其亚群的变化,为正确评价T淋巴细胞在Mp感染中的作用提供理论依据。Mp滴鼻感染小鼠,在感染后不同时间点采集标本,流式细胞术检测感染鼠外周血中T淋巴细胞数量变化,ELISA测定脾细胞上清中TGF-β1、IL-10含量。结果表明,Mp感染不同时间点外周血中CD4+T淋巴细胞数量变化不明显或呈下降趋势。CD4+/CD8+比值下降。初次感染和再次感染的第3天、第7天,TGF-β1、IL-10表达水平升高。肺炎支原体感染对CD4+T淋巴细胞有一定抑制作用,外周血TGF-β1、IL-10的表达与脾细胞中CD4+T淋巴细胞数量变化成反比。提示TGF-β1可能参与了对Th1细胞的负调控作用。Dynamic changes of T cells quantity and their subgroups in Mycoplasma pneumoniae（ Mp） infected mice were observed to properly evaluate the role of T cells in Mp infection and to provide theoretical basis for Mp infection study. BALB / c mice were intranasally infected with Mp whole cells. Samples were collected at different time points after the infection. The changes of peripheral blood of T lymphocyte quantity of the infected mice was counted by flow cytometry. Cytokines of spleen cells supernatant were assessed by ELISA. CD4＋T-lymphocyte count of the peripheral blood had no difference or assumed a declining drift at the different time points after the Mp infection. CD4＋/ CD8＋ratio was decreased. The levels of TGF-β1,IL-10 were increased on day 3 and 7 of initial infection and re-infection.Therefore,Mp infection had a certain inhibition against CD4＋T cells,and the expression of TGF-β1 and IL-10 was in inverse proportion to the change of CD4＋T cell count of spleen cells. These suggested that TGF-β1 may participate in the negative regulation function of Th1 cells.

关 键 词：肺炎支原体 CD4+T淋巴细胞 动态变化 TGF-Β1 

分 类 号：Q939.93[生物学—微生物学] R37[医药卫生—病原生物学]