1株大熊猫肠道纤维素降解菌的分离鉴定及其酶学性质  

Isolation of Cellulose Degradable-Bacteria from Giant Panda's Intestines and Its enzymatic Characterization

作　　者：赵珊[1] 吕雯婷[1] 刘杰[1] 宋亮丽 张海峰[1] 孙小琴[1] 颜其贵[1,2] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]四川农业大学动物疫病与人类健康四川省重点实验室,四川雅安625014

出　　处：《微生物学杂志》2015年第1期73-78,共6页Journal of Microbiology

基　　金：国家级创新创业训练计划项目(201310626009);四川省检验检疫局课题(SK201305)

摘　　要：以羧甲基纤维素钠为唯一碳源,利用刚果红染色法,从大熊猫粪便内筛选具有降解纤维素能力的菌株,并研究其酶学特性。分离获得1株酶活力较高的菌株A1,通过形态学和BD PhoenixTM-100全自动细菌鉴定仪鉴定为蜡样芽胞杆菌(Bacillus cereus)。菌株A1最适生长条件和酶活力测定表明,其最适生长温度为37℃,Na Cl浓度为0.5%,p H值为7.0,内切葡聚糖苷酶、外切葡聚糖苷酶、β-葡萄糖苷酶和总酶活的最大值分别为0.139、0.074、0.126、0.108 5 IU/m L。丰富了大熊猫肠道纤维素降解菌的种类,为后续研究大熊猫如何消化利用竹纤维提供了菌源。Using sodium carboxymethylcellulose as unique carbon source and Congo red staining method,cellulose degradable bacterial strains were isolated from giant panda＇s fecal samples,and studied on their enzymatic characteristics. Strain A1 with high enzyme activity was isolated,it was identified as Bacillus cereus morphologically and through BD PhoenixTM-100 full-automatic bacteria identification instrument. Growth condition and enzymatic activity tests showed that the optimal growth temperature of the strain was 37 ℃. 0. 5% Na Cl and p H at 7. 0 the maximum activity of endo-glucanase was at 0. 139 IU / m L,exo-glucanase 0. 074 IU / m L,beta-glucosidase 0. 126 IU / m L and the total ernzymatic activity was 0. 108 5 IU / m L respectively. This research enriched the species of cellulose degrading-bacteria in giant panda＇s intestines and strain A1 provided strain source for the following up study on how the giant panda digest and utilize bamboo fiber.

关 键 词：大熊猫 纤维素降解菌 蜡样芽胞杆菌 

分 类 号：Q93[生物学—微生物学] Q781