百草枯农药对小鼠血液中血细胞参数的影响  

The Effects of Paraquat Pesticide on Blood Cell Parameters in Mice

作　　者：彭蔷[1] 夏敏[2] 孙涛[1] 刘丽达[1] 李蓉[2] 王林[1] 车茜[1] 朱明华[1] 

机构地区：[1]四川省疾病预防控制中心,成都610041 [2]崇州市人民医院

出　　处：《职业卫生与病伤》2015年第2期116-119,共4页Occupational Health and Damage

基　　金：四川省科技支撑项目(2012SZ0102)

摘　　要：目的观察百草枯农药急性经口染毒对小鼠的毒性反应和血细胞参数的影响。方法 SPF级昆明种小鼠60只,随机分为百草枯农药染毒6 h、1 d、3 d和7 d和对照组共5组,百草枯农药60 mg/kg.BW一次经口灌胃染毒,对照组使用蒸馏水灌胃。观察动物的体重和死亡数等中毒表现,并测定血液中14项血细胞参数。结果百草枯农药染毒动物体重下降、活动减少、毛发蓬松、呼吸困难,死亡率为16.67%。百草枯农药染毒3 d组与对照组比较,雌鼠的淋巴细胞百分比(LYMPH%)降低(F=2.96,P<0.05),单核细胞百分比(MONO%)增高(F=3.83,P<0.05),差异有统计学意义(P<0.05)。结论百草枯农药60 mg/kg经口染毒对小鼠产生明显的毒性反应症状,染毒后3 d血液中淋巴细胞百分比降低,单核细胞百分比增高。Objective To observe the toxic effects and effects on blood cell parameters in mice exposed to paraquat pesticide with acute oral route. Methods Sixty specific pathogen free（ SPF） Kunming mice were divided randomly into 5groups,i. e. 4 groups exposed to paraquat pesticide in 6 h,1 d,3 d and 7 d and one control group. The paraquat pesticide was administered by gavage at 60 mg / kg. BW dose in the 4 experimental groups,while distilled water was administered in control group. The indexes of toxicity,such as the animal body weight,were observed,and the number of deaths and 14 blood cell parameters were measured as well. Results The body weight and physical activity were both decreased,and fluffy fur,breathing difficulties were all exhibited in mice poisoned by paraquat pesticide; the mortality was16. 67%. The lymphocyte percentage of female mice was statistically decreased（ F = 2. 96,P〈0. 05）,and the monocyte percentage of female mice was statistically increased（ F = 3. 83,P 〈0. 05） in paraquat pesticide exposed for 3d group than that in the control group（ P〈0. 05）. Conclusion The obvious signs of toxicity in mice could be produced by paraquat pesticide administered at 60 mg / kg dose in oral route,and blood lymphocyte percentage declination,monocyte percentage increasing are both exhibited after 3 days of poisoning.

关 键 词：百草枯 农药 毒性 血细胞参数 

分 类 号：R114[医药卫生—卫生毒理学]