枸骨中芦丁和槲皮素含量测定  

Determination of rutin and quercetin in Ilex cornuta Lindl.et Paxt.

作　　者：李艳芝[1] 姜静静[1] 

机构地区：[1]济宁医学院药学院,山东日照276826

出　　处：《济宁医学院学报》2015年第2期96-98,共3页Journal of Jining Medical University

基　　金：2013年日照市应用技术研究与开发计划项目(编号:2013JHSZ017)

摘　　要：目的采用高效液相色谱法测定枸骨不同部位（嫩叶、老叶、枝、青果、红果）及不同采收期枸骨叶中芦丁和槲皮素的含量。方法采用C18色谱柱（250mm×4.6mm,5μm）,流动相为甲醇-0.5%磷酸水溶液（47∶53）,流速为0.7ml/min,检测波长为356nm,柱温25℃,测定枸骨中芦丁和槲皮素含量。结果芦丁和槲皮素线性范围分别为0.52-5.2μg和0.34-3.4μg,相关系数r均为0.9999,平均回收率分别为98.6%和99.2%,RSD分别为1.70%和1.91%（n=6）。结论枸骨不同部位及不同采收期枸骨叶中芦丁和槲皮素含量有明显差异。Objective Using HPLC to determin the contents of flavonoids（rutin and quercetin）in Ilex cornuta Lindl.et Paxt.and provide the scientific basics of quality control and reasonable application.Methods The contents of rutin and quercetin in Ilex cornuta Lindl.et Paxt.were analyzed by C18 column（250 mm×4.6 mm,5μm）using methanol-0.5% aqueous solution of phosphoric acid（47：53）as mobile phase at the flow rate of 0.7 ml/min and UV detection was at 356 nm.Results Rutin and quercetin respectively showed good linear relationships at the range of 0.52 - 5.2μg（r=0.9999）and 0.34-3.4μg（r=0.9999）.The recovery rates were 98.6% and 99.2%（n=6）while RSD1.70% and 1.91%（n=6）.Conclusion The contents of rutin and quercetin in different parts of Ilex cornuta Lindl.et Paxt and the leaves harvesting in different months are different.

关 键 词：枸骨 芦丁 槲皮素 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]