粗毛栓菌原生质体UV诱变漆酶高产菌株的选育  

UV-Mutagenesis and screening of high laccase-producting strains from trametes gallica protoplasts

作　　者：赵颖[1] 孙崇然[1] 王宇飞[1] 张莹[1] 王晓丽[1] 朱友双[1] 

机构地区：[1]济宁医学院生物科学学院,山东日照276826

出　　处：《济宁医学院学报》2015年第2期106-109,共4页Journal of Jining Medical University

基　　金：山东省自然科学基金联合专项(编号:ZR2014CL002);2014山东省高等学校青年骨干教师国内访问学者项目;济宁医学院青年科研基金资助项目(编号:JYQ2011KM012);济宁医学院博士科研启动基金(编号:094201)

摘　　要：目的以粗毛栓菌Trametes hirsuta lg-9为出发菌株进行原生质体的UV诱变,以期获得漆酶高产菌株。方法优化粗毛栓菌Trametes hirsuta lg-9原生质体的制备方法,并摸索该菌株紫外诱变条件。结果筛选出2株漆酶高产菌株JM-70S3和JM-70S8,分别是出发菌株Trametes hirsuta lg-9漆酶产量的93.57倍和257.24倍。结论原生质体紫外诱变是粗毛拴菌获得漆酶高产菌株的有效方法。Objective To obtain the high laccase-producing strains,we used the method of UV-mutagenesis to the Trametes hirsuta lg-9 protoplasts.Methods We optimized the protoplast preparation method of bacteria Trametes hirsuta lg-9,and groped for the strain ultraviolet mutagenesis condition.Results JM-70S3 and JM-70S8 were screened out,which produced laccase 93.57 and 257.24 higher than the original strains.Conclusion The UVmutagenesis is an effective method to obtain the high laccase-producing strains of Trametes hirsuta.

关 键 词：漆酶 粗毛拴菌 原生质体 紫外诱变 

分 类 号：Q815[生物学—生物工程]