CCL3、CCL4下调表达和重症继发性肺结核病理损伤的关系  

Correlation between down-regulated expression of CCL3,CCL4 and pathological damage in severe secondary pulmonary tuberculosis patients

作　　者：苏瑾文[1] 刘艳华[2] 杨秉芬[2] 程小星[2] 

机构地区：[1]解放军309医院结核病研究所ICU,北京100091 [2]解放军309医院结核病研究室

出　　处：《山西医科大学学报》2015年第4期300-304,共5页Journal of Shanxi Medical University

基　　金：国家传染病重大科技专项基金资助项目(2013ZX10003-006-003-001)

摘　　要：目的研究趋化因子CCL3和CCL4下调表达与重症继发性肺结核病理损伤的相关性。方法用基因表达谱芯片对5例重症、5例轻症继发性肺结核和5例健康对照进行差异基因的筛选;用实时荧光定量PCR测定20例重症、20例轻症,患者和8例健康对照外周血单个核细胞CCL3、CCL4相对表达量;酶联免疫吸附法测定20例重症、20例轻症和20例健康对照CCL3、CCL4在血浆中的蛋白表达量;用方差分析和非参数检验统计方法判断组间比较的统计学差异。结果芯片筛选重症与轻症组比较的差异基因显示CCL3、CCL4表达下调,下调比值分别为0.153 1和0.122 7。实时荧光定量PCR结果、酶联免疫吸附法均显示重症组与轻症组相比CCL3、CCL4表达下调(均P<0.05)。结论重症继发性肺结核患者CCL3、CCL4表达下调,可能是促进重症继发性肺结核肺结构破坏的因素之一。Objective To investigate the correlation between down-regulated expression of CCL3,CCL4 and pathological injury in severe secondary pulmonary tuberculosis（ TB）.Methods Differentially expressed genes were screened with gene expression chips in 5cases of severe secondary TB,5 cases of mild secondary TB,and 5 healthy controls.The relative transcription levels of CCL3,CCL4 were detected in peripheral blood mononuclear cells in 20 cases of severe TB,20 cases of mild TB and 8 healthy controls by real timePCR.The serum protein levels of CCL3 and CCL4 were detected by ELISA in 20 cases of severe TB,20 cases of mild TB and 20 healthy controls.Analysis of variance and non-parametric tests were used for data analysis among the groups.Results Gene chip analysis for differential expressed gene selection showed that CCL3,CCL4 were decreased by 0.153 1 and 0.122 7 folds respectively in severe TB group compared with mild TB group.The results of real time-PCR and ELISA both verified that CCL3 and CCL4 were downregulated in severe TB group compared with mild TB group（ P 〈0.05）.Conclusion CCL3 and CCL4 are down-regulated in severe secondary TB patients.The two chemokines may involve in the lung tissue damage of severe secondary TB.

关 键 词：重症肺结核 CCL3 CCIA 免疫反应 

分 类 号：R521[医药卫生—内科学]