含活性炭纤维敷料类产品的体外细胞毒性研究  

In vitro Cytotoxicity of Activated Carbon Fiber Dressing Products

作　　者：尹玉霞[1,2] 侯丽[1,2] 王鸾鸾[1,2] 李海心[1,2] 郑雯雯[1,2] 

机构地区：[1]山东省医疗器械产品质量检验中心 [2]山东省医疗器械生物学评价重点实验室,济南250101

出　　处：《生物医学工程研究》2015年第1期64-66,共3页Journal Of Biomedical Engineering Research

摘　　要：活性炭纤维因其高效吸附特性而广泛应用于医疗卫生领域。我们设计不同的浸提液制备方法,通过MTT法评价该浸提液对小鼠成纤维细胞L929活性及增殖的影响,以寻找适合评价含活性炭产品体外细胞毒性的浸提方法。结果显示,由于活性碳纤维的强吸附性,相对于传统的含血清细胞培养基直接作为浸提介质,采用无血清培养基浸提,试验前在浸提液中加入10%血清更适合评价含活性炭产品的体外细胞毒性。Activated carbon fiber is widely used in medical and health field because of its high adsorption properties. L- 929 mouse fibroblasts were evaluated by MTT assay after incubation with a series of extracts to find suitable extraction method in vitro cytotoxicity for activated carbon products. The results show that due to the strong adsorption of activated carbon fiber,compared with traditional serum containing cell culture medium as extraction vehicle,a method using serum- free medium as extraction vehicle with adding 10% serum medium to the extracts is more suitable for evaluation of cytotoxicity in vitro for activated carbon products.

关 键 词：活性炭纤维 敷料 浸提介质 体外细胞毒性 

分 类 号：R318[医药卫生—生物医学工程]