雷公藤内酯醇对糖尿病肾病大鼠肾内Glut-1、Glut-4表达的影响  

Influence of triptolide on the expression of Glut-1,Glut-4 in kidney of diabetic nephropathy rats

作　　者：尤丽菊[1] 郭辉[1] 李宜川[1] 

机构地区：[1]商丘医学高等专科学校,河南商丘476000

出　　处：《实用药物与临床》2015年第4期390-393,共4页Practical Pharmacy and Clinical Remedies

基　　金：2011年河南省教育厅自然科学研究计划项目(2011C310003)

摘　　要：目的探讨雷公藤内酯醇对糖尿病肾病大鼠肾内Glut-1、Glut-4表达的影响。方法将45只大鼠按体重随机分为正常对照组(C组,13只)和糖尿病模型组(32只)。模型组腹腔注射链脲菌素(STZ)52 mg/kg,1次/d,连续5 d;正常对照组仅腹腔注射等体积上述缓冲液。完成STZ注射72 h后,尾静脉采血测空腹血糖,并同时测尿糖,血糖≥16.7 mmol/L、尿糖+++以上者列入糖尿病观察对象。将造模成功的28只雄性大鼠,按体重、空腹血糖随机分为DM模型组对照(DM组,14只)、雷公藤内酯醇组(TP组,14只)。结果 DM组、TP组第4、第8周的24 h尿m ALB定量较C组明显增加(P<0.01),且DM组第8周高于第4周(P<0.01),TP组第8周较第4周明显降低(P<0.01)。DM组系膜细胞及上皮细胞Glut-1的表达较正常对照组均明显增加(P<0.05),Glut-4的表达均有所减少(P<0.05)。与DM组比较,TP组系膜细胞及上皮细胞Glut-1的表达降低(P<0.01);Glut-4在系膜细胞的表达升高(P<0.05),在上皮细胞的表达变化不明显(P>0.05)。结论雷公藤内酯醇能明显降低糖尿病肾病大鼠肾小球系膜细胞及上皮细胞内的Glut-1含量,增高Glut-4在肾小球系膜细胞的表达。Objective To discuss the influence of triptolide on the expression of Glut-1,Glut-4 in kidney ofdiabetic nephropathy rats. Methods 45 rats were randomly divided into tw o groups according to weight： normal control group（ group C,n = 13） and diabetic model group（ n = 32）. Rats in model group were intraperitoneally injected streptozotocin（ STZ） 52 mg / kg for 5 d continuously; rats in group C were given intraperitoneal injection of equal volume of the buffer solution. At 72 h after the injection,the levels of fasting blood glucose and urine glucose were detected,and the rats with blood glucose≥16. 7 mmol / L,urine glucose more than ＋ ＋ ＋ were included as the diabetes objects. The 28 male rats（ successful model） were randomly divided into DM model group（ DM group,n = 14） and triptolide group（ TP group,n = 14）. Results At the 4th and 8th weeks,the 24 h urinary m ALB level in DM group and TP group significantly increased than that of group C（ P〈0. 01）,the m ALB in DM group at the 8th week was higher than that at the 4th week（ P〈0. 01）,but the m ALB in TP group at the 8th week reduced（ P〈0. 01）. The expression of Glut-1 in mesangial and epithelial cells in DM group increased more significantly than that of group C（ P〈0. 05）,and the expression of Glut-4 reduced（ P〈0. 05）. Compared with DM group,the expression of Glut-1 in mesangial and epithelial cells of group PT was significantly low er（ P〈0. 01）,the expression of Glut-4 in mesangial cells increased（ P〈0. 05）,no significant difference was found in the expression of epithelial cell（ P〈0. 05）. Conclusion Triptolide can significantly reduce the content of Glut-1 in glomerular mesangial and epithelial cells of diabetic nephropathy rats,increase the Glut-4 expression in glomerular mesangial cells.

关 键 词：雷公藤内酯醇 糖尿病肾病 大鼠 GLUT-1 GLUT-4 

分 类 号：R285.5[医药卫生—中药学]