甲病毒载体的研究进展  

Review on Alphavirus-based Vectors

作　　者：龚文芝 刘灿[1] 张东东[1] 宁宜宝[1] 

机构地区：[1]中国兽医药品监察所,北京100081

出　　处：《中国兽药杂志》2015年第4期65-69,共5页Chinese Journal of Veterinary Drug

摘　　要：甲病毒属于披膜病毒科,是一类有包膜的单股正链RNA病毒。近些年来,国内外有关甲病毒载体的研究非常活跃,其已被用于疫苗研究、基因治疗以及分子生物学等研究领域。基于甲病毒载体的构造原理,将其分为三类,分别为复制完全型载体、复制缺陷型载体和DNA/RNA载体。就这三类载体的改造过程进行了探讨,并对这三类载体的特点及其应用研究进展等方面进行了阐述,以期为开发出更安全、更有效的甲病毒载体提供参考。Alphaviruses belong to the family of Togaviridae, which are encapsulated by a capsid protein and possess single strand plus RNA. In recent years, the domestic and foreign research on alphavirus-based vectors is active and the alphavirus-based vectors have been used for vaccine research, gene therapy and molecular biology research. Based on the structural principle, the alphavirus vectors are classified into three main types：replication-competent vector, replication-deficient vectors and DNA-layered vector. In this review, the transformation, characteristics and application of three different alphavirus-based vectors are discussed in order to provide references for the development of safer, more effective alphavirus-based vectors.

关 键 词：甲病毒 复制子载体 高水平表达 

分 类 号：S852.659.6[农业科学—基础兽医学]