人卵巢干细胞体外培养方法的初步探讨  

Preliminary investigationofovarianstemcellsin-vitrocultivation

作　　者：赵琰誉[1] 赵诗艺 刘英[1] 

机构地区：[1]首都医科大学附属北京妇产医院生殖医学科,北京100026

出　　处：《中国优生与遗传杂志》2015年第4期108-110,F0002,共4页Chinese Journal of Birth Health & Heredity

基　　金：国家自然科学基金项目(81471520);国家留学基金项目(2011911033);北京市自然科学基金项目(5122015);北京市卫生系统高层次卫生技术人才培养项目资助

摘　　要：目的初步探讨人卵巢干细胞体外培养方法,为进一步研究提供实验基础。方法取本院因非卵巢癌因素切除全子宫及双附件患者的部分卵巢组织,胶原酶消化,体外培养,观察细胞集落生长情况,通过碱性磷酸酶染色及免疫荧光检测Oct-4表达情况,鉴定集落的多能性。结果共收集10例围绝经期患者卵巢组织,培养成功5例,碱性磷酸酶染色呈紫蓝色,免疫荧光法鉴定集落Oct-4阳性。结论该卵巢标本处理方法可培养出细胞集落,经初步鉴定碱性磷酸酶染色阳性,表达多能干细胞标志物Oct-4,可以作为卵巢干细胞进一步研究的培养方法。Objective：To investigatein-vitro cultivation of human ovarianstem cells and provide an experimentalbasis for furtherresearch. Methods：Part of ovarian tissue collected from patients underwent hysterectomy and bilateral salpingooophorectomy due toa non-ovarian-cancer factor,were treated with collagenase and cultured in-vitro for observation. The colony pluripotency was detected by immunofluorescencestaining for Oct-4 expression and alkaline phosphatasedying. Results：The colonies presented purple blue after alkaline phosphatase（AKP）stainingand Oct-4（＋）after immunofluorescence.Conclusion：Colonies obtained through this method were preliminarily identified as AKP（＋）and Oct-4（＋）.This method could be used for further study of ovarianstem cells.

关 键 词：卵巢 干细胞 体外培养 

分 类 号：R714.8[医药卫生—妇产科学]