丹瓜方干预Apo E^-/-糖尿病模型小鼠脑组织IGF-1表达研究  

Dangua recipe on intervening IGF-1 in encephalon of mice with Apo E^-/-Diabetes Mellitus

作　　者：颜群芳 陈依楚 黄苏萍[1] 衡先培[2] 陈燕[3] 李亮[2] 王思梅[1] 康文倩 陈超云[1] 叶梦捷 郑沪琳 

机构地区：[1]福建中医药大学,福州350122 [2]福建中医药大学附属人民医院,福州350004 [3]杭州市余杭区第一人民医院,浙江杭州311100

出　　处：《吉林中医药》2015年第4期404-406,共3页Jilin Journal of Chinese Medicine

基　　金：国家自然科学基金资助项目(81173179);福建省科技重点项目(2014Y0063);福建省自然科学基金(2012J01392)

摘　　要：目的探讨丹瓜方对Apo E^-/-糖尿病模型小鼠脑组织胰岛素样生长因子-1(insulin-like growth factor-1,IGF-1)表达水平的影响,以探究丹瓜方防治糖尿病脑病的部分机制。方法以链脲佐菌素(STZ)对Apo E^-/-小鼠塑造糖尿病模型。将成模后的小鼠按体质量分层,依照随机数字表随机分为模型组、丹瓜方组、吡格列酮组、联合组,每组8只。选取血糖正常的同龄同品系C57小鼠8只作为对照组。分组灌胃干预12周。ELISA法测定IGF-1表达。结果与C57组IGF-1(19.89±0.95)ng/m L比较,其余各组均明显降低(P<0.01);丹瓜方组(14.31±0.99)ng/m L和联合用药组IGF-1水平(11.58±0.92)ng/m L明显高于模型组(7.25±0.42)ng/m L;丹瓜方组IGF-1水平明显高于联合用药组(P<0.01)。结论通过适度提升糖尿病鼠脑组织中IGF-1表达,可能是丹瓜方防治糖尿病脑病的部分机制。Objective To research the effects of Dangua Recipe（ DGR） on glycolipid metabolism and its expression level of IGF-1 in encephalon in Apo E^-/- mice with Diabetes Mellitus,thus revealing its partial mechanisms for preventing and treating diabetic encephalopathy. Methods Diabetic model was molded with streptozotocin peritoneally injected to Apo E^-/-mouse. The modeled mice were stratified by body weight,and then randomly divided into the model group（ MOD）,the Dangua Recipe group（ DGR）,the combination group（ COM）,and the pioglitazone group（ PIO） according to the random number table,8 in each group. Another 8 euglycemial C57 mice of the same ages and strain were recruited as the control group. The interventions lasted for 12 weeks by gastrogavage. The level of IGF-1 in encephalon was analyzed with ELISA. Results IGF-1（ 19. 89 ± 0. 95） ng / m L in C57 group was higher than all others along（ P〈0. 01）; IGF-1 in DGR（ 14. 31 ± 0. 99） ng / m L and COM（ 11. 58 ± 0. 92） ng / m L group was higher than MOD（ 7. 25 ± 0. 42） ng / m L group; IGF-1 in DGR group was significantly higher when compared with COM group（ P〈0. 01）. Conclusion Moderately increasing the expression of IGF-1 in mice with diabetes may be part of Dangua Recipe＇s mechanism of prevention and treatment of diabetic encephalopathy.

关 键 词：丹瓜方 糖尿病脑病 胰岛素样生长因子-1 丹参 瓜蒌 川芎 郁金 

分 类 号：R285.5[医药卫生—中药学]