柴胡聪耳汤逆转庆大霉素诱发大鼠耳毒性的抗氧化机制研究  

Experimental study on Radix Bupleuri Conger Decoction partly reversing rat gentamicin ototoxicity via antioxidation mechanism

作　　者：王树春 丁国臣[2] 韩磊[2] 

机构地区：[1]武警沈阳指挥学院门诊部,辽宁沈阳110034 [2]武警辽宁总队医院,辽宁沈阳110034

出　　处：《武警后勤学院学报（医学版）》2015年第3期179-181,共3页Journal of Logistics University of PAP（Medical Sciences）

摘　　要：【目的】观察柴胡聪耳汤对庆大霉素致耳聋模型大鼠的抗氧化作用的影响,探索其抗氧化作用机制。【方法】30只SPF级SD大鼠,随机分为3组:空白对照组、模型对照组、柴胡聪耳汤组。采用庆大霉素腹腔注射的方法诱导药物性耳聋大鼠模型,通过柴胡聪耳汤中药汤剂灌胃对模型大鼠进行干预,采用听觉脑干诱发电位听觉脑干诱发电位(auditory brainstem response,ABR)阈值对模型进行评价,并对各组大鼠血清中超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、过氧化氢酶(catalase,CAT)含量进行检测。【结果】与空白对照组比较,模型对照组大鼠ABR阈值显著升高(P<0.01),与模型对照组比较,柴胡聪耳汤组大鼠ABR阈值显著降低(P<0.01);与空白对照组比较,模型组大鼠血清SOD、CAT含量显著降低,而MDA含量显著升高(P<0.01);与模型对照组比较,柴胡聪耳汤组大鼠血清中SOD、CAT含量显著升高,而MDA含量显著下降(P<0.01)。【结论】柴胡聪耳汤对庆大霉素诱发的大鼠耳毒性具有部分逆转作用,这种逆转作用可能是通过调节血清中SOD、CAT和MDA水平而实现的。[Objective}To observe the antioxidation effect of Radix Bupleuri Conger Decoction on deaf rats by gentamicin, and explore its antioxidation mechanism. [ Methods ]Thirty SPF SD rats were randomly divided into 3 groups： control group, model group and Radix Bupleuri Conger Decoction group. Rat model of pharmic hearing loss was induced by intraperitoneal injection of gentamicin and interfered with Radix Bupleuri Conger Decoction by mouth injection. The models were evaluated by ABR threshold and the contents of the SOD, MDA and CAT in serum of each group were detected. [ Results] Compared with control group, ABR threshold in model group increased significantly obviously（P〈0.01）. While compared with model group, ABR threshold in Radix Bupleuri Conger Decoction group decreased obviously （P〈0.O1）. In contrast to control group, the contents of SOD and CAT were lower while MDA content was higher in model control group（P〈0.01）. In contrast to model group, the contents of SOD and CAT were higher while MDA content was lower in Radix Bupleuri Conger Decoction group（P〈0.01）. [Conclusion] Radix Bupleuri Conger Decoction can partly reverse ototoxicity of gentamicin on model rats, probably through adjusting the levels of SOD, CAT and MDA in serum.

关 键 词：庆大霉素 耳聋 听觉脑干诱发电位 超氧化物歧化酶 丙二醛 过氧化氢酶 

分 类 号：R289.58[医药卫生—方剂学]