上海市闵行区0～15岁儿童水痘-带状疱疹病毒抗体水平调查研究  

Serological survey of varicella-zoster virus among children aged 0-15 years in Minhang district of Shanghai

作　　者：杜艳[1] 余峰[1] 张莉萍[1] 金宝芳[1] 王烨[1] 梅克雯[1] 李捷[1] 蒋露芳[2] 

机构地区：[1]上海市闵行区疾病预防控制中心,上海201101 [2]复旦大学公共卫生学院

出　　处：《中国预防医学杂志》2015年第4期252-256,共5页Chinese Preventive Medicine

基　　金：上海市卫生局基金资助项目(20114085)

摘　　要：目的 了解新生儿出生后至水痘减毒活疫苗（varicella attenuated live vaccine,VarV）接种后不同年限水痘-带状疱疹病毒抗体（varicella-zoster virus antibody,VZV-IgG）水平变化状况,为VarV免疫策略的制定提供依据。方法 采集505份血清样本,其中小于12月龄（无水痘患病史）样本114份,大于12月龄（均有一剂次VarV接种史）样本391份,用酶联免疫吸附试验（enzyme-linked immunosorbent assay,ELISA）定量检测VZV-IgG。数据采用Excel 2007录入、作图,并用SPSS 18.0进行分析比较。结果 小于12月龄儿童VZV-IgG阳性率为35.09%（40/114）,几何平均浓度（geometric mean concentration,GMC）为54.38IU/L。6月龄后VZV-IgG阳性率及GMC下降幅度较大,至9月龄降至最低。不同月龄组间阳性率差异有统计学意义（χ2=25.498,P〈0.01）,并有随着月龄增长而下降的趋势。大于12月龄儿童VZV-IgG阳性率为74.42%（291/391）,GMC为330.49IU/L。不同性别、户籍、疫苗品种VZV-IgG阳性率差异无统计学意义（χ2=4.626,P=0.099;χ2=1.869,P=0.393;χ2=5.526,P=0.063）。对不同品种VarV接种后不同年限VZV-IgG定性检测结果分析显示,国产VarV接种组VZV-IgG阳性率最高为11~年组,为88.89%（8/9）;其次是1~年组,为83.78%（31/37）,最低是7~年组,为48.72%（19/39）,国产VarV接种组不同年限VZV-IgG阳性率经过检验差异有统计学意义（χ2=14.298,P=0.014）;进口VarV接种组VZV-IgG阳性率最高为11~年组,阳性率为95.45%（21/22）;其次是9~年组,为83.33%（20/24）,最低是5~年组,为71.79%（28/39）,进口VarV接种组不同年限VZV-IgG阳性率经过统计学检验差异无统计学意义（χ2=5.992,P=0.307）。对不同品种VarV接种后不同年限VZV-IgG定量检测结果分析显示,国产VarV接种后GMC最高为11~年组,为751.92IU/L。其次是9~年组,为450.70IU/L。最低是7~年组,为133.35IU/L;进口VarV接种后GMC最高为11~年组,为550.12IU/L。其次是1~年组,为450.70IU/L。最低是5~�Objective To understand the dynamic change of antibody against varicella-zoster virus （VZV-IgG） among healthy children after varicella attenuated live vaccine （VarV） inoculation. Methods A total of 505 serum samples were collected, 114 samples were from children under 12 months old without varicella history, and 391 were from children older than 12 months old with at least one VarV vaccination. Enzyme linked immunosorbent assay （ELISA） was used to determine VZV-IgG antibody level. SPSS 18.0 software was used for data analysis. Results The VZV-IgG positive rate was 35.09~ among children under 12 months old with geometric mean concentration （GMC） of 54.38 IU/L. Both VZV IgG positive rate and GMC decreased tremendously after 6 months of age, and reached the minimum level at 9 months of age. The positive rates were significantly different among children in age groups （X^2 = 25. 498, P= 0. 000）, which tended to decrease along with age. The positive rate of VZV-IgG among children older than 12 months was 74.42% （291/391） with GMC of 330.49 IU/L. There were no significant differences of antibody positive rates among children with different gender, registered residence and vaccine type （X^2 = 4. 626, P = 0. 099 ; X^2 = 1. 869, P = 0. 393 ; X^2 = 5. 526, P=0. 063）. Meanwhile, the antibody positive rate was the highest of 88. 89% among children in 11- age group who received domestically manufactured VarV, and the lowest of 48.72% in 7- age group children, the difference was significant （X^2= 14. 298, P= 0.014）. However, the antibody positive rates were similar among children in different age groups who received imported VarV vaccines （X^2 = 5. 992, P= 0. 307）. The antibody level was the highest of 751.92 IU/L in 11- year age group and the lowest of 133.35 IU/L in 7 year age group among those who were vaccinated with domestic vaccine and in 5- year age group among children inoculated with imported vaccine （265.63 IU/L）. A U-shaped distribution of GMC was identified with the bottom fa

关 键 词：儿童 水痘-带状疱疹病毒抗体 酶联免疫吸附试验 

分 类 号：R446.6[医药卫生—诊断学]