左旋门冬酰胺酶对jurkat细胞株增殖凋亡影响的研究  

The study of effects of L-asparaginase on jurkat Cells＇ proliferation and apoptosis

作　　者：郭玉玲[1] 卢愿[1] 孙立荣[1] 仲任[1] 李学荣[1] 

机构地区：[1]青岛大学附属医院血液儿科,山东青岛266003

出　　处：《医药前沿》2015年第7期31-34,共4页Journal of Frontiers of Medicine

摘　　要：目的：研究左旋门冬酰胺酶对急性T淋巴细胞白血病jurkat细胞株生长的影响。方法：在倒置相差显微镜下观察jurkat细胞经左旋门冬酰胺酶处理前后的生长状态，Gimsa染色观察L—Asp处理前后jurkat细胞的形态变化。通过琼脂糖凝胶电泳检测经不同浓度左旋门冬酰胺酶处理后的jurkat细胞的DNA片段。用流式细胞术检测Jurkat细胞经不同浓度门冬酰胺酶处理48小时后的凋亡率及死亡率。结果：终浓度为1IU／m1、2．5IU／ml、5IU／ml、10IU／ml L-Asp均可诱导jurkat细胞发生凋亡和死亡，L-Asp终浓度为2．5IU／ml时jurkat细胞凋亡率最高，死亡率随L-ASP浓度增高而升高；凋亡细胞基因组DNA片段化断裂，凝胶电泳可见梯状条带。结论：左旋门冬酰胺酶可以诱导jurkat细胞发生凋亡，其凋亡细胞DNA发生片段化断裂，凋亡率与左旋门冬酰胺酶浓度有关。Objective To study the effects of L-asparaginase on the proliferation and apoptosis of human acute T-cell leukemia Jurkat cells. Method The morphological changes were observed under a microscope with Gimsa stained cells. DNA fragments were detected by Agarose gel electrophoresis. Cell apoptosis was analysed by Flow cytometry. Results The jurkat cells, treated with L-Asp in the concentration of 1IU/ml, 2.5IU/ml, 5IU/ml, 101U/ml for 48h, can be induced apoptosis and death. The jurkat cells＇ apoptosis rate was highest when treated with the concentration of L-Asp was 2.5IU/ml. The jurkat cells＇ mortality rates increased with L-Asp concentration riseing. DNA ladder can be showed by DNA electrophoresis after the jurkat cells were treated with 1IU/ml, 2.5IU/ml, 5IU/ml, 10IU/ml L-ASp. Conclusion The apoptosis of Jurkat cells can be induced by L-ASp. Apoptotic DNA occurs gragmentation. Apoptosis rate is associated with Concentration of L-Asp.

关 键 词：左旋门冬酰胺酶 白血病 细胞凋亡 JURKAT细胞株 

分 类 号：R392[医药卫生—免疫学]