A Study on the Sensibility of Embryogenic Suspension Cells of Anthurium andraeanum to Two Antibiotics  

作　　者：Bo YU Genfa ZHU Yingbo SUN 

机构地区：[1]Environmental Horticulture Institute,Guangdong Academy of Agricultural Sciences / Guangdong Key Lab of Ornamental Plant Germplasm Innovation and Utilization

出　　处：《Agricultural Biotechnology》2015年第2期8-11,共4页农业生物技术（英文版）

基　　金：Supported by Special Fund of President of the Guangdong Academy of Agricultural Sciences(201217)

摘　　要：In this study,embryogenic cell aggregates obtained from the established embryogenic cell suspension culture system of Anthurium andraeanum‘Alabama’were used as experimental materials to investigate the effects of kanamycin and hygromycin on survival rate of cell aggregates,somatic embryogenesis and plantlet regeneration of A.andraeanum.According to the results,at the embryonic cell propagation stage,lethal doses of kanamycin and hygromycin to embryogenic cell aggregates of A.andraeanum were 200 and 60 mg/L,respectively;at the differentiation stage,either 150 mg/L kanamycin or 40 mg/L hygromycin could inhibit somatic embryogenesis;either 100 mg/L kanamycin or 20 mg/L hygromycin could inhibit plantlet regeneration.These results provided important reference for further studies of transgenic A.andraeanum.In this study,embryogenic cell aggregates obtained from the established embryogenic cell suspension culture system of Anthurium andraeanum‘Alabama’were used as experimental materials to investigate the effects of kanamycin and hygromycin on survival rate of cell aggregates,somatic embryogenesis and plantlet regeneration of A.andraeanum.According to the results,at the embryonic cell propagation stage,lethal doses of kanamycin and hygromycin to embryogenic cell aggregates of A.andraeanum were 200 and 60 mg/L,respectively;at the differentiation stage,either 150 mg/L kanamycin or 40 mg/L hygromycin could inhibit somatic embryogenesis;either 100 mg/L kanamycin or 20 mg/L hygromycin could inhibit plantlet regeneration.These results provided important reference for further studies of transgenic A.andraeanum.

关 键 词：Anthurium embryogenesis somatic transgenic regeneration embryonic suspension Araceae callus breeding 

分 类 号：S682.14[农业科学—观赏园艺]