Determination of the Concentration of Chlorella vulgaris Beij by Visible Spectrophotometer  

作　　者：Zhixu ZHAO Jingyang PAN Liangbo WU Wei ZHANG Junsheng XU Yugui CHEN Zhide FU 

机构地区：[1]Gansu Endangered Animal Protection Centre,State Forestry Administration [2]Qianxuesen Sand Industry Central Laboratory [3]School of Aerospace,Tsinghua University

出　　处：《Agricultural Biotechnology》2015年第2期54-55,58,共3页农业生物技术（英文版）

基　　金：Supported by the Spark Program of Gansu Provincial Science and Technology Department(5HS066-A91-015-01)

摘　　要：[Objective]This study aimed to provide technical parameters for large-scale production and industrial development of Chlorella vulgaris Beij.[Method]By using 721 visible spectrophotometer,the transmitted light intensity of different concentrations of C.vulgaris liquid under different wavelengths was measured to normalize the absorbance data,thus determining the optimal wavelength for the growth of C.vulgaris and the concentration of C.vulgaris liquid under the optimal wavelength.The regression equation of absorbance at the optimal wavelength and C.vulgaris concentration was obtained to derive the formula between equivalent concentration C and transmitted light intensity I,thus calculating the equivalent concentration of 0.25-fold C.vulgaris liquid under different transmittance.[Result]The transmitted light intensity of C.vulgaris reached the peak at 670 nm,indicating that 670 nm is the optimal wavelength for measuring C.vulgaris concentration.The regression equation between absorbance and C.vulgaris concentration was y=-1.479 7＋0.987x,with a correlation coefficient of 0.991,suggesting a high correlation between these two factors.The formula of equivalent concentration C and transmitted light intensity I was C=-ln（I/I0）/LK,where L indicates the optical path length（cuvette width）and K indicates the average cross-sectional area of C.vulgaris cells.[Conclusion]It is feasible to investigate indirectly the concentration of C.vulgaris with a spectrophotometer,which can be used to determine the optimal dehydration time of C.vulgaris.[Objective]This study aimed to provide technical parameters for large-scale production and industrial development of Chlorella vulgaris Beij.[Method]By using 721 visible spectrophotometer,the transmitted light intensity of different concentrations of C.vulgaris liquid under different wavelengths was measured to normalize the absorbance data,thus determining the optimal wavelength for the growth of C.vulgaris and the concentration of C.vulgaris liquid under the optimal wavelength.The regression equation of absorbance at the optimal wavelength and C.vulgaris concentration was obtained to derive the formula between equivalent concentration C and transmitted light intensity I,thus calculating the equivalent concentration of 0.25-fold C.vulgaris liquid under different transmittance.[Result]The transmitted light intensity of C.vulgaris reached the peak at 670 nm,indicating that 670 nm is the optimal wavelength for measuring C.vulgaris concentration.The regression equation between absorbance and C.vulgaris concentration was y=-1.479 7＋0.987x,with a correlation coefficient of 0.991,suggesting a high correlation between these two factors.The formula of equivalent concentration C and transmitted light intensity I was C=-ln（I/I0）/LK,where L indicates the optical path length（cuvette width）and K indicates the average cross-sectional area of C.vulgaris cells.[Conclusion]It is feasible to investigate indirectly the concentration of C.vulgaris with a spectrophotometer,which can be used to determine the optimal dehydration time of C.vulgaris.

关 键 词：vulgaris Chlorella calculating dehydration visible absorbance transmitted transmittance sectional feasible 

分 类 号：Q94-33[生物学—植物学]