单粒蓖麻种子毒蛋白含量测定方法的研究  

Reseach on the method of determination of ricin content of a castor seed

作　　者：雷雪[1,2] 黄凤兰[2,3] 孟凡娟[1] 罗蕊[2] 陈晓凤[2] 赵永[2] 李跃[2] 孙华军[2] 

机构地区：[1]东北林业大学生命科学院,黑龙江哈尔滨150036 [2]内蒙古民族大学生命科学院,内蒙古通辽028000 [3]内蒙古自治区高校蓖麻产业工程技术研究中心,内蒙古通辽028000

出　　处：《内蒙古农业科技》2015年第2期5-8,11,共5页Inner Mongolia Agricultural Science and Technology

基　　金：国家自然科学基金项目(30760123;31160290);国家农业部公益性行业项(201003057);内蒙古自治区高等学校"青年科技英才支持计划"(NJYT-14-A10);市校合作项目(SXZD2012018);内蒙古自治区高校蓖麻产业工程技术研究中心开放基金项目(BMYJ2011009)

摘　　要：以"通蓖5号"单粒蓖麻种子为研究对象,利用BCA试剂盒、UVwin5紫外软件V5.1.0、Band Scan蛋白定量分析软件相结合的方法,优化了单粒蓖麻种子的粗蛋白提取过程,并对单粒蓖麻种子中毒蛋白含量进行测定。结果如下,优化后的单粒蓖麻种子粗蛋白提取过程中缓冲液A的加入量为3m L/g,硫酸铵饱和度为70%,沉淀时间是12h,溶解沉淀所需加入缓冲液A为3m L。经测定,单粒蓖麻种子平均质量为0.2415g,其中含粗蛋白10.8908mg,毒蛋白4.1154mg,毒蛋白所占百分比为37.79%。The single ＂Tongbi5＂ castor seed was the study object in this paper.Aiming for optimizing extraction pro-cess of crude protein,a variety of methods of which BCA kit,UVwin5 ultraviolet software（v5.1.0） and Band Scan protein quantitative analysis software were used.The results were as follows,in the optimized extraction process of crude protein of a castor seed,the amount of buffer A is 3m L/g,time of precipitation with ammonium sulfate（70% satu-ration） is 12 h,and the amount of buffer A to dissolve precipitation is 3m L.It was found by measurement that the average quality of a castor seed is 0.2415 g,the average content of crude protein is 10.8908 mg.Ricin makes up crude protein by 37.79 and content of ricin is 4.1154 mg on average in a castor seed.

关 键 词：蓖麻种子 毒蛋白 磷酸盐提取法 

分 类 号：S565.6[农业科学—作物学]