辽宁杨叶片高频植株再生体系的建立  

Establishment of A Highly Efficient Plant Regeneration System of P.×Liaoningensis

作　　者：赵鑫闻 彭儒胜 赵继梅 

机构地区：[1]辽宁省杨树研究所,辽宁盖州115200

出　　处：《中国农学通报》2015年第10期13-16,共4页Chinese Agricultural Science Bulletin

基　　金：辽宁省科学事业公益研究基金项目"黑杨花粉管通道导入外源DNA及其细胞学机理研究"(2011005007)

摘　　要：为了提高辽宁杨的抗逆性及扩大它的适生区域,本研究以辽宁杨叶片为外植体材料,通过对附加不同浓度BA和NAA的MS培养基的筛选,建立了辽宁杨叶片高频植株再生体系,其不定芽诱导率及生根率都达到100%,辽宁杨最佳不定芽分化培养基配方为MS+BA 0.3 mg/L+NAA 0.05 mg/L,生根培养基配方为1/2MS+IBA 0.3 mg/L,为辽宁杨的抗性改良及分子育种实践奠定了基础。In order to enhance its resistance to cold, disease and pests and enlarge its growing area, a highlyefficient plant regeneration system with 100% shoot regeneration and rooting rate from leaf explants of P.×Liaoningensis in vitro was established by comparing different concentrations of BA and NAA on MS medium,and it was concluded that the best medium for shoot regeneration was MS ＋BA 0.3 mg/L ＋ NAA 0.05 mg/L,and the best medium for rooting was 1/2MS＋IBA 0.3 mg/L. The results of this study will contribute to theimprovement of the resistance of poplar and the study of the molecular breeding.

关 键 词：辽宁杨 叶片 不定芽 再生 

分 类 号：S792.11[农业科学—林木遗传育种] S722.37[农业科学—林学]