信阳地区5种叶蝉的种类鉴定及亲缘关系分析  

Identification and Genetic Relationship of Five Leafhopper Species in Xinyang Area

作　　者：乔利[1,2] 秦道正[2] 张丽[2] 李伯辽 夏明聪[3] 李慧龙 卢兆成 

机构地区：[1]河南省信阳市农业科学院,河南信阳464000 [2]西北农林科技大学植物保护学院,陕西杨凌712100 [3]河南省农业科学院植物保护研究所,郑州450002

出　　处：《中国农学通报》2015年第10期176-181,共6页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"中国重要林业害虫小绿叶蝉族的分类暨系统发育研究"(31270689)

摘　　要：为了解不同种叶蝉的寄主选择习性及种类分布,通过室内解剖鉴定和COI与Cytb基因遗传距离分析,明确信阳地区4种寄主植物上的叶蝉种类及亲缘关系。结果表明:茶园的叶蝉主要为小贯小绿叶蝉;花生和麻田的优势种群为板井小绿叶蝉,其中麻田还有棉叶蝉分布;桃树上的叶蝉分别为凯小绿叶蝉和桃新叶蝉。3种小绿叶蝉间的亲缘关系较近,桃新叶蝉与其他4种叶蝉的遗传距离均较远。笔者从分子水平上分析了叶蝉的亲缘关系,为进一步研究叶蝉的寄主转移及种群分布提供参考。In order to know the host choosing habits and species distribution of leafhoppers in Xinyang area,the species and genetic relationship of the leafhoppers in four hosts were identified by anatomy identification inlaboratory using genetic distance analysis of COI gene and Cytb gene. The results showed that the leafhoppersin tea plantation were mainly Empoasca onukii Matsuda. Empoasca sakaii Dworakowska was the dominantpopulation in kenaf and peanut field, and Amrasca biguttula Ishida was also found in kenaf filed. There weremainly two kinds of leafhoppers, which were Empoasca kaicola Dworakowska and Singapora shinshana Matsumura, on peach trees. The genetic distance analysis of COI gene and Cytb gene showed that geneticrelationship of the 3 kinds of green leafhoppers was close. Genetic distance of Singapora shinshana Matsumurawas far away from others. The study of genetic relationship of five leafhoppers in different hosts on themolecular level provided reference for the further study of host transfer and species distribution of leafhoppers.

关 键 词：半翅目 叶蝉科 COI CYTB 形态学 系统发育 

分 类 号：S435.711[农业科学—农业昆虫与害虫防治]