基于SRAP分子标记的剑豆遗传多样性分析  

Genetic diversity of Canavalia ensiformis(L.)DC.accessions revealed by SRAP markers

作　　者：刘明骞[1] 陈丽君[2] 丁美美[1] 欧阳昆唏[1] 惠文凯 李俊成[1] 陈晓阳[1] 

机构地区：[1]华南农业大学林学院/广东省森林植物种质创新与利用重点实验室,广州510642 [2]华南农业大学教学科研基地管理中心,广州510642

出　　处：《中国农业大学学报》2015年第2期58-66,共9页Journal of China Agricultural University

基　　金：广州市科技计划项目(2010Z1-E241);农业部"948"项目(2011-Z50)

摘　　要：采用相关序列扩增多态性（Sequence-related amplified polymorphism,SRAP）分子标记方法,对收集到的来源于11个国家的19个剑豆种群进行遗传多样性分析。结果表明：1）在扩增出的274条清晰条带中,具有多态性的有144条,占总数的52.6%;2）28对引物的多态性指数（Polymorphism information content,PIC）介于0.16~0.43,平均值为0.28;3）剑豆总遗传多样性指数（Ht）为0.285 6,基因流（Nm）为0.065 6;4）通过分子方差分析（Analysis of molecular variance,AMOVA）发现,剑豆种群间差异占总差异的88%,种群内差异占总差异的12%,种群间差异为剑豆差异的主要来源;5）主坐标分析（Principal coordinates analysis,PCoA）和基于遗传距离的聚类分析表明,19个剑豆种群可分为5个类群,每个类群均有不同的表型。Canavalia ensiformisis an under-exploited legume that has been used as forage,green manure and cover crop.It can potentially be used in numerous other ways,including nutraceuticals and phytopharmaceuticals.In this study,sequence-related amplified polymorphism（SRAP）markers were used to assess the genetic diversity and relationship of 19 C.ensiformis accessions originated from 11 countries.The results showed that：1）In total,274 clear bands were amplified and 144fragments（52.6%）were polymorphic;2）The polymorphism information content（PIC）values ranged from 0.16 to 0.43,with an average of 0.28;3）The total genetic diversity（Ht）was 0.285 6,the estimate of gene flow（Nm）was 0.065 6;4）The results from the analysis of molecular variance（AMOVA）revealed that the most significant variation,88% of the total variation,occurred among accessions and 12% was attributed to within accession variation;5）The cluster analysis and principal coordinates analysis（PCoA）produced similar results,whereby the 19 C.ensiformis were divided into five clusters,each cluster was composed of different accessions and had different phenotypic traits.This study provided the theoretical basis for biodiversity studies and breeding programs of Canavalia ensiformis.

关 键 词：剑豆 相关序列扩增多态性 遗传多样性 

分 类 号：Q37[生物学—遗传学]