丹参减压提取与常规提取比较研究  

Comparison of vacuum and conventional extraction of Salvia miltiorrhiza Bge.

作　　者：马然[1,2] 赵伟[1,2] 段文娟[2] 王晓[2] 刘伟[2] 刘峰[2] 刘建华[2] 

机构地区：[1]山东师范大学生命科学学院,山东济南250014 [2]山东省分析测试中心,山东济南250014

出　　处：《山东科学》2015年第2期26-30,2,共5页Shandong Science

基　　金：山东省自然科学基金三院联合基金(ZR2014YL005);国家自然科学基金(81102749)

摘　　要：本文对比了减压法与常规法提取中药丹参得到的丹酚酸B含量、蛋白质含量、可溶性糖含量、干膏得率、提取液透光率以及DPPH自由基清除率。优选减压提取的最佳工艺条件为:乙醇浓度50%、料液比1:10、真空度0.07 MPa、温度为50℃、提取时间1.0 h。在相同的溶剂条件下,丹参采用减压提取与加热回流提取,丹酚酸B的提取率及DPPH自由基清除率相当,高于超声提取和温浸提取;减压提取法的干膏得率、蛋白质和可溶性糖的含量高于超声提取和温浸提取,但是低于加热回流提取。综合比较,减压法具有丹酚酸B提取率高、提取液澄清和杂质少等特点,优于常规提取工艺。We compared the contents of Salvianolicacid B, protein and soluble sugar, light transmittance and DPPH free radical scavengiffg rate of Salvia miltiorrhiza Bge. between vacuum extraction and conventional extraction. Optimum conditions of vacuum extraction were ethanol concentration of 50%, solid to liquid ratio of 1： 10, vacuum degree of 0.07 MPa, temperature of 50 ℃ and extraction time of 1. 0 hour. For identical extraction agents, extraction rates of Salvianolic acid B and DPPH free radical scavenging rate were equal for vacuum extraction and heat reflux extraction, greater than those of ultrasonic extraction and warm immersion extraction. The extract yield, contents of protein and soluble sugar of vacuum extraction were higher than those of ultrasonic extraction and warm immersion extraction, but lower than those of heat reflux extraction. Vacuum extraction of Salvia miltiorrhiza Bge. has high extraction rate of Salvianolic acid B, clear extracting solution and less impurity, superior to conventional extraction.

关 键 词：丹参 减压提取 SALVIA miltiorrhiza Bge. 

分 类 号：R284.2[医药卫生—中药学]