慢性丙型肝炎患者中CD4^+CD25^+CD127^-调节性T细胞的表达及意义  

Expression and significance of CD4^+CD25^+CD127^-regulatory T Cells in patients with chronic hepatitis C viral infection

作　　者：韩荔芬[1,2] 林国贤[3] 陈怡[2] 陈清 邱荣仙[3] 郑玲[2] 

机构地区：[1]福建医科大学第一临床医学院,福建福州350005 [2]福州市传染病医院,福建福州350025 [3]福建莆田学院附属医院,福建莆田351100 [4]福州市苍霞社区服务中心,福建福州350009

出　　处：《临床和实验医学杂志》2015年第7期562-565,共4页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨CD4^+CD25^+CD127^-调节性T细胞在慢性丙型肝炎(HCV)患者中的表达及其临床意义。方法收集HCV患者67例,并根据是否吸毒分为吸毒丙肝组35例和非吸毒丙肝组32例;健康体检人员32例作为对照。采用流式细胞仪检测患者外周血中CD^4+CD25^+CD127^-调节性T细胞的表达;应用实时荧光定量PCR的方法检测其HCV RNA含量。结果慢性HCV患者中的吸毒感染组与非吸毒感染组的外周血中CD4+CD25+CD127-调节性T细胞在CD4+T细胞中所占比率分别为:3.84±1.95%、4.44±1.67%,明显低于对照组6.10±1.65%(P<0.01);而吸毒组与非吸毒组慢性HCV患者血液CD4^+CD25^+CD127^-调节性T细胞的表达值相比差异无统计学意义;HCV患者抗病毒治疗前血液CD4^+CD25^+CD127^-调节性T细胞的表达值明显低于治疗后和正常对照组(P<0.01)。结论 HCV患者治疗前患者外周血CD4^+CD25^+CD127^-调节性T细胞的表达值较正常对照组明显降低,而在治疗有效后升高,说明CD4^+CD25^+CD127^-调节性T细胞参与了HCV患者的机体免疫过程,而CD4^+CD25^+CD127^-调节性T细胞在外周血中的表达水平可能成为监测HCV患者体内细胞免疫状态和机体抗病毒免疫应答的指标。Objective To explore the expression of CD4^＋CD25^＋CD127^-regulatory T Cells from patients with chronic hepatitis C viral infection,and to evaluate its clinical significance. Methods 67 patients with HCV and 32 healthy volunteers were included in this study. The proportion of CD4^＋CD25^＋CD127^-regulatory T cells in peripheral blood was detected by using flow cytometry. HCV RNA detection in the peripheral blood of HCV patients were done by real- time fluorescent quantitative PCR. Results Comparing with healthy controls,drug abusing and non- drug abusing HCV- infected patients in peripheral blood had a lower percentage of CD4^＋CD25^＋CD127^-regulatory T cells. The proportion of CD4^＋CD25^＋CD127^-regulatory T cells to CD4＋T cells in peripheral blood from drug abusing and non- drug abusing HCV- infected patients（ 4. 44% ± 1. 67%） account for（ 3. 84% ± 1. 95%） and（ 4. 44% ± 1. 67%） respectively,which was significantly lower than those from healthy controls（ 6. 10% ± 1. 65%）（ P〈0. 01）. However,there was no significant difference between the drug abusing and non- drug abusing HCV- infected patients（ P〈0. 05）; The expression of CD4^＋CD25^＋CD127^-regulatory T cells in HCV group before antiviral treatment was evidently lower than that in treated HCV group and control group（ P〈0. 01）. Conclusion The expression of CD4^＋CD25^＋CD127^-regulatory T cells in the antiviral treatment HCV patients was evidently higher than those of untreated HCV patients,which explained that the regulatory T cell possibly hold a role in the activation of the immune response to HCV infection. In addition,the expression level of CD4＋CD25＋CD127-regulatory T cells in peripheral blood may be monitoring the patient＇s body cell immune status and become the index of body anti- virus immune response.

关 键 词：丙型肝炎 CD4^+CD25^+CD127^-调节性T细胞 流式细胞术 

分 类 号：R512.63[医药卫生—内科学]