中药材生物质还原制备银纳米颗粒及其抑菌活性研究  

Study on preparation of silver nanoparticles by biomass reduction of traditional Chinese medicinal materials and their antibacterial activity

作　　者：蔡伟[1] 马月[2] 方杰[2] 吴方[3] 余陈欢[2] 

机构地区：[1]浙江医药高等专科学校中药学院,浙江宁波315100 [2]浙江省医学科学院浙江省实验动物与安全性研究重点实验室,浙江杭州310013 [3]浙江中医药大学药学院,浙江杭州310053

出　　处：《中草药》2015年第7期977-981,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81202977);浙江省新苗人才计划(2010R410055)

摘　　要：目的利用中药材提取液来还原Ag+获得相应的银纳米材料,并对其抑菌活性进行研究。方法利用大黄等15种中药材提取液还原制备了银纳米溶液,采用激光粒度仪(HPPS)、透射电镜(TEM)、紫外可见分光光度计(UV)测定了银纳米颗粒的尺寸和分布。采用纸片琼脂扩散法和倍比稀释法,测定了银纳米颗粒的抑菌作用。结果大黄、制首乌、香薷水提液还原制备的银纳米颗粒得率和反应速率较理想。制备所得的银纳米粒径为100 nm左右,均匀性好,分散性好,对金黄色葡萄球菌和大肠杆菌的生长均有明显的抑制作用,其中,以制首乌-银纳米抑菌作用最强。结论中药材作为天然生物还原剂,可用于银纳米颗粒的制备。Objective To use the extracts from traditional Chinese medicinal materials（TCMM） for reduction of Ag＋ to obtain the corresponding silver nanomaterials and study its antibacterial activity. Methods Nano-silver solution was prepared by Rhei Radix et Rhizoma and other 15 kinds of TCMM extracts, the size and distribution of silver nanoparticles were studied using HPPS, TEM, and UV. The antibacterial activities of silver nanoparticles were investigated by disk agar diffusion method and double dilution method. Results Preparation rate and reaction rate of silver nanoparticles prepared with the water extract of Rhei Radix et Rhizoma, Polygoni Multiflori Radix Praeparata, Moslae Herba were ideal. Prepared silver nanoparticles with diameter about 100 nm, good uniformity, and good dispersibility have the obvious inhibitory effect on the growth of Staphylococcus aureus and Escherichia coli. Among them, Polygoni Multiflori Radix Praeparata-silver nano has the strongest bacteriostatic effect. Conclusion TCMM as a natural bio-reducing agents, can be used to prepare the silver nanoparticles.

关 键 词：银纳米 绿色合成 中药材 抑菌作用 大黄 制首乌 香薷 

分 类 号：R283.6[医药卫生—中药学]