六味地黄方醇沉工艺研究  

Study on alcohol precipitation technology of Liuwei Dihuang Prescription

作　　者：韦迎春[1,2] 闫明[1,2] 胡军华[1,2] 李雪峰[1,2] 吴云[1,2] 萧伟[1,2] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中药制药过程新技术国家重点实验室,江苏连云港222001

出　　处：《中草药》2015年第8期1161-1166,共6页Chinese Traditional and Herbal Drugs

基　　金：科技部重大新药创制(2013ZX09402203)

摘　　要：目的通过对六味地黄方醇沉工艺合理化研究,得到稳定的、可产业化的醇沉工艺。方法以马钱苷、芍药苷、莫诺苷、甘露三糖、多糖质量分数、相对分子质量分布及浸出物等为评价指标,对乙醇加入方式、离心方式及是否加入乙醇洗涤工艺进行考察,筛选出最佳醇沉工艺。结果醇沉工艺中以体积计算加入乙醇的量较合理;采用乙醇洗涤法可有效完成多糖部位与醇沉上清液的分离;改进工艺后,多糖浸出物质达标,苷类、甘露三糖的质量分数达标。结论经优化后的乙醇醇沉工艺具有易操作、可控性好、重复性好的特点,可以开展产业化推广。Objective Through studying the rationalization of alcohol precipitation process of Liuwei Dihuang Prescription to obtain the stability alcohol precipitation process which could be used to promote industrialization.Methods It screens the most reasonable alcohol precipitation process taking the contents,the relative molecular weight distribution,and the extract of loganin,peoniflorin,morroniside,manninotriose,and polysaccharide as the evaluation index to investigate the way of adding ethanol,centrifugation,,and whether to carry out the ethanol washing process.Results The alcohol precipitation process is reasonable by calculating the alcohol volume method.The polysaccharides and alcohol precipitation supernatant could be efficiently separated by ethanol washing method.The polysaccharide extract and the contents of glycosides and manninotriose were up to the standard after the process improved.Conclusion The optimized alcohol precipitation process is easy operation with good controllability and repeatability.It is worthy of popularization and application of industrialization.

关 键 词：六味地黄方 中试工艺 生产工艺 多糖 醇沉 

分 类 号：R284.2[医药卫生—中药学]