斑马鱼药物代谢模型的适用性研究进展  

Advances in study on applicability of drug metabolism model in zebrafish

作　　者：宁青[1] 韦英杰[1] 王丹丹[1] 贾晓斌[1] 谢林[2] 

机构地区：[1]中国中医科学院江苏分院,江苏省中医药研究院,国家中医药管理局中药口服释药系统重点研究室,江苏南京210028 [2]南京中医药大学附属江苏省中西医结合医院,江苏南京210028

出　　处：《中草药》2015年第8期1231-1236,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30973978);江苏省自然科学基金资助项目(BK20141507);江苏省六大人才高峰(2013-YY006;2010-WS-071);2014年度江苏省第四期"333工程"科研资助项目(BRA2014348)

摘　　要：根据相关研究及国外有关文献资料,对斑马鱼药物代谢的实验方法、代谢类型及适用性进行归纳和总结。斑马鱼较成功地模拟了哺乳动物Ⅰ相代谢、Ⅱ相代谢、肠道菌群代谢及多途径整体代谢,适用于微量单体化合物及其组合物的在体代谢研究,有利于代谢产物的简单、高效富集,具简单、高效、低成本、化合物用量少(毫克级)等优势,是一种早期、快速预测微量成分在体代谢的极具前景的模式生物模型。According to relative research and foreign references,we summarized the experimental method,drug metabolism type,and applicability of drug metabolism model in zebrafish.Zebrafish successfully simulated the mammals in phase I metabolism,phase II metabolism,intestinal bacteria metabolism,and multiway in vivo metabolism.Zebrafish is suitable for in vivo metabolism of trace monomeric compounds and their combinations,and is benefit for the efficient enrichment of metabolites in simple way.It has the advantages of simplity,high efficiency,low cost,and less amount of compound（a few mg） etc.It is a very prospect organisms model for early and rapid prediction of in vivo metabolism of trace elements.

关 键 词：斑马鱼 Ⅰ相代谢 Ⅱ相代谢 肠菌代谢 药物代谢 

分 类 号：R285[医药卫生—中药学]