依达拉奉对肝损伤模型小鼠的肝保护作用研究  

Study on the Protective Effects of Edaravone on Model Mice with Liver Injury

作　　者：黄学桂 包旻 赵冰封 

机构地区：[1]安徽省妇幼保健院药学部,合肥230001

出　　处：《中国药房》2015年第13期1785-1787,共3页China Pharmacy

摘　　要：目的:研究依达拉奉对对乙酰氨基酚致肝损伤模型小鼠的保护作用。方法:取小鼠随机分为正常对照(生理盐水)组、模型(生理盐水)组、阳性对照(联苯双酯150 mg/kg)组和依达拉奉高、中、低剂量(10、5、2.5 mg/kg)组,每组10只,尾iv给予相应药物,连续14 d。末次给药后除正常对照组外,其余各组小鼠ip给予对乙酰氨基酚0.3 g/kg复制肝损伤模型。复制模型16 h后,各组小鼠眼球取血检测血清中谷氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、白细胞介素2(IL-2)、IL-6水平;处死小鼠后取肝组织,检测其中脂质过氧化物(LPO)、丙二醛(MDA)、超氧化物歧化酶(SOD)水平并观察其病理学变化。结果:与正常对照组比较,模型组小鼠血清中ALT、AST、IL-6水平增加,IL-2含量降低,肝组织中LPO和MDA含量增加,SOD活性降低,差异均具有统计学意义(P<0.01)。与模型组比较,依达拉奉高、中、低剂量组小鼠血清中ALT活性降低;依达拉奉高、中剂量组小鼠血清中IL-2含量增加,AST活性和IL-6含量降低,肝组织中LPO和MDA含量降低,SOD活性增强,差异均具有统计学意义(P<0.05)。与模型组比较,依达拉奉高、中剂量组小鼠肝细胞变性、炎性细胞浸润减少,坏死程度显著减轻。结论:依达拉奉对对乙酰氨基酚致肝损伤模型小鼠有明显的保护作用,其机制可能与抗氧化及增强免疫有关。OBJECTIVE： To study the protective effects of edaravone on model mice with liver injury induced by paracetamol. METHODS： Mice were randomly divided into normal control group （normal saline）, model group （normal saline）, positive con- trol group （bifendate 150 mg/kg） and edaravone high-dose, medium-dose and low-dose groups （10, 5 and 2.5 mg/kg）, 10 for each. They were given corresponding drugs in tail for 14 d, iv. After the last administration, except for normal control group, mice in other groups were given paracetamol 0.3 g/kg to reproduce the models of liver injury, ip. After reproducing models for 16 h, the levels of alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, interleukin-2 （IL-2） and IL-6 in all groups were determined by eyeball blood. Liver tissue was collected after executed, the levels of lipid peroxide （LPO）, malondialdehyde （MDA） and superoxide dismutase （SOD） were determined and the pathological changes were observed. RESULTS： Compared with normal control group, the levels of ALT, AST and IL-6 in model group were increased, the content of IL-2 were decrease, the contents of LPO and MDA in liver tissue were increased and the activities of SOD was decreased, with significant difference （P〈0.01）. Com- pared with model group, the activities of ALT in serum of mice in edaravone high-dose, medium-dose and low-dose groups were decrease, the content of IL-2 in serum of mice in edaravone high-dose and medium-dose groups were increased, the activities of AST and content of IL-6 was decreased; the contents of LPO and MDA in liver tissue were decrease and the activities of SOD was increased, with significant difference （P〈0.05）. Compared with model group, the liver cells＇ degeneration and inflammatory cell infiltration of mice in edaravone high-dose and medium-dose groups were decrease and the necrosis degree was obviously relieved. CONCLUSIONS： Edaravone has obvious prospective effects on the model mice with liver injury induced b

关 键 词：依达拉奉 对乙酰氨基酚 肝损伤 机制 小鼠 

分 类 号：R965[医药卫生—药理学]