基于炮制学理论初探知母润肠通便作用的有效成分  

Exploring effective components of laxative effect of Anemarrhenae Rhizoma based on Chinese herbal processing theory

作　　者：雷霞[1] 张婕[1] 李媛[1] 王秋红[1] 薛娟[1] 苏晓琳[1] 王长福[1] 夏永刚[1] 匡海学[1] 

机构地区：[1]教育部北药基础与应用研究重点实验室,黑龙江省中药及天然药物药效物质基础研究重点实验室,黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中国中药杂志》2015年第7期1283-1286,共4页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2012CB504708);国家自然科学基金项目(81274103);全国百篇优秀博士学位论文作者专项资金(201367)

摘　　要：该实验对知母盐炙前后水煎液通便作用及化学成分进行比较研究,通过观察生、盐知母水煎液对便秘大鼠的通便作用,采用超高效液相-质谱(UPLC-Q-TOF-MS)技术对知母盐炙前后化学成分进行比较,苯酚硫酸法测定其总糖含量。盐知母水煎液低、中、高剂量组使大鼠粪便粒数显著增加,与同等剂量生知母相比有显著差异(P<0.05);总离子流图比较无明显差异,但总糖含量明显高于生知母。结果表明,知母盐制品在同等剂量时通便作用明显强于生品,与其盐制后甘味的作用增强有关,是其滋阴降火作用增强的具体体现;糖类化合物可能是其滋阴降火作用的物质基础,也是知母甘味的物质基础。Anemarrhena asphodeloides processed by salt and raw product was compared including both chemical composition and laxative function in order to find the possible active substance to cure constipation. Processed and raw Anemarrhenae laxative effect on experimental constipation models was observed as well as chemical composition using UPLC-MS technology and the total sugar content was determined by phenol sulfuric acid method. Processed Anemarrhenae water extract improved excrement more than raw which has significant difference compared with the blank group（P〈0.05）. On the other hand, the total ion flow spectrum showed no significant difference in most substance, but the total sugar content was significantly higher than raw product. Anemarrhenae ancient be recognized benefitting for draining body water in traditional Chinese medicine which has been lost in modern books because it is manifested as excellent laxative effect not diuretic effect. Saccharides carbohydrate may have closely relationship with this magically effect.

关 键 词：知母 利水 盐炙知母 润肠通便 

分 类 号：R283[医药卫生—中药学]