同时制备克级高纯度酪醇、大花红天素及红景天苷的工艺研究  

Simultaneously preparation of grams of high purity tyrosol,crenulatin and salidroside from Rhodiola crenulata

作　　者：罗鑫[1,2] 王雪晶[1,2] 李石平[1,2] 张桥[1,2] 赵祎武[1,2] 黄文哲[1,2] 王振中[1,2] 萧伟[1,2] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中药制药过程新技术国家重点实验室,江苏连云港222001

出　　处：《中国中药杂志》2015年第7期1300-1304,共5页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2013ZX09402203)

摘　　要：酪醇、大花红天素及红景天苷3种化合物均是红景天的主要活性成分,具有广泛的药理活性。该实验首次从大花红景天中同时分离出克级高纯度的酪醇、大花红天素及红景天苷,红景天经70%乙醇提取后,以3种化合物的收率为指标,对树脂工艺进行优化筛选,再利用硅胶柱分离纯化,最终同时得到克级高纯度的酪醇、大花红天素及红景天苷,经HPLC测定,3种化合物的纯度均超过98%。该方法工艺简洁、操作简单、有机溶剂用量少、收率及重复率都很高,适合3种化合物的同时制备。Tyrosol, crenulatin and salidroside are the main active constituents of Rhodiola crenulata, with extensive pharmacological activities. In the study, grams of high purity tyrosol, crenulatin and salidroside were simultaneously separated from R. crenulata by the first time. Firstly, R. crenulata was extracted by 70% alcohol. Then, with the yields of three compounds as the index, the macroporous resin was optimized. At last, grams of high purity tyrosol, crenulatin and salidroside were isolated by D-101 macroporousresin, purified by column chromatography. Detected by HPLC, the purity of three compounds were higher than 98%. This method has the advantages of simple process and operation, less dosage of organic solvent, highly yield and reproducibility, suitable for the simultaneously preparation of tyrosol, crenulatin and salidroside.

关 键 词：同时制备 克级 高纯度 酪醇 大花红天素 红景天苷 

分 类 号：R283.6[医药卫生—中药学]