RP-HPLC-UV同时测定白木香叶中二苯甲酮和酮类成分  

Simultaneous determination of benzophenones and xanthone in leaves of Aquilaria sinensis by RP-HPLC-UV

作　　者：夏芳[1,2] 吕海宁[1] 姜勇[1] 屠鹏飞[1] 

机构地区：[1]北京大学药学院天然药物及仿生药物国家重点实验,北京100191 [2]太极医药研究院,重庆400118

出　　处：《中国中药杂志》2015年第7期1342-1346,共5页China Journal of Chinese Materia Medica

基　　金：国家“重大新药创制”科技重大专项(2012ZX09301002-002-002,2012ZX09304-005)

摘　　要：利用高效液相色谱法(RP-HPLC-UV),同时测定白木香叶中的4个主要成分,包括3个二苯甲酮(iriflophenone 3-C-β-D-glucoside,iriflophenone 3,5-C-β-D-diglucoside,iriflophenone 2-O-α-L-rhamnoside)和1个口山酮(mangiferin)的含量;采用Agilent ZORBAX SB-C_(18)色谱柱(4.6 mm×250 mm,5μm),乙腈-0.1%磷酸水梯度洗脱,流速为1.0 mL·min^(-1),检测波长为285nm,柱温为25℃。通过上述条件对15批白木香叶中的4个主要成分进行了含量测定;上述4个待测物在上述色谱条件下能够达到基线分离,标准曲线线性关系良好(R^2>0.999 0),精密度和稳定性符合分析方法学要求,加样回收率为98.80%~101.39%(RSD<3.0%),其含量分别在0.41~14.48 mg,0.72~3.85 mg,4.30~29.07 mg,0.24~5.06 mg;该分析方法准确可靠,重复性好,能实现对白木香叶的质量控制,有利于该资源的综合利用开发。This study is to develop a sensitive method by using reversed-phase high performance liquid chromatography coupled with UV detector （HPLC-UV） to simultaneously determine four bioactive compounds, iriflophenone 3-C-β-D-glucoside, iriflophenone 3,5-C-β-D-diglucoside, mangiferin, and iriflophenone 2-O-α-L-rhamnoside in the leaves of Aquilaria sinensis. An Agilent Zorbax SB-C18 column （4.6 mm×250 mm, 5 μm） was used, and the gradient elution was performed with mobile phase of 0.1% aqueous phosphoric acid and acetonitrile at a flow rate of 1 mL·min-1. The detection wavelength was 280 nm, and the column temperature was 25℃. The four marker compounds were well separated with good linearity （R2 〉0.999 0）, precision, stability and repeatability. The recovery rates were in the range of 98.80%-101.39%. For 15 branch of the leaves, the contents of iriflophenone 3-C-β-D-glucoside, iriflophenone 3,5-C-β-D-diglucoside, mangiferin, and iriflophenone 2-O-α-L-rhamnoside were between 0.41-14.48, 0.72-3.85, 4.30-29.07, 0.24-5.06 mg, respectivley. This method is precise, accurate and reliable, which provides an efficient way for the quality control of the leaves of A. sinensis. This will promote the comprehensive usage of this plant.

关 键 词：白木香叶 二苯甲酮 (口山)酮 含量测定 

分 类 号：R284.1[医药卫生—中药学]