当归药材补血活性的定量测定  

Bioassay for enrich-blood bioactivity of Agelicae Sinensis Radix

作　　者：王晓晓[1] 张丽宏 李曦 王野 荣祖元 危红萍 宋启瑞 吕光华[1] 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137 [2]四川省食品药品检验检测院,四川成都610097

出　　处：《中国中药杂志》2015年第7期1381-1387,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81073044;J1310034-02)

摘　　要：当归为常用的补血药,以阿魏酸等已知化学成分为指标难以有效评价其质量。为此,根据生物检定的原理,建立了定量测定当归药材补血活性的生物检测方法。当归药材依次用乙醇和水提取,以合并后的总提物为供试品进行小鼠补血活性试验;用环磷酰胺和盐酸苯肼联合建立小鼠血虚模型;以红细胞数为补血活性的评价指标,用环孢菌素A标定当归的补血活性。再根据量反应平行线(3·3′)法计算供试品的补血活性。结果表明,环孢菌素A和当归总提物补血活性的可靠性检验结果成立;与模型组相比,当归总提取物具有显著的补血活性(P<O.05)。环孢菌素A在给药剂量0.035~0.56 g·kg^(-1)与红细胞数增加值呈良好的线性关系(r=0.998 4,n=5)。供试品补血活性重复测定的RSD为6.15%(n=6),可信限率为26.68%(n=6)。5份从甘肃、四川、云南收集的当归药材样品的性状特征不同,其补血活性也不同(21.95~44.16 U·g^(-1))。表明建立的方法可准确测定当归药材的补血活性,评价其质量。Danggui, Agelicae Sinensis Radix, is a widely used Chinese herb to enrich blood, but its quality cannot be effectively assessed by the known chemical markers such as ferulic acid, ligustilide, polysaccharides, etc. A new bioassay was therefore developed to quantify the Enrich-Blood Bioactivity （EBB） for the quality assessment of Danggui raw materials. Danggui sample was first extracted with ethanol and water, respectively. Then the ethanolic extract and water extract were mixed as a test sample to quantify the amount of EBB by mice experiment. The blood deficiency mode in mice was developed by intraperitoneal injecting cyclophospharmide and phenylhdrazine hydrochloride. The quantity of red blood cell was chosen as EBB marker. Cyclosporine A was chosen as a control substance. EBB in analytes was quantified by the amount reaction of parallel line analysis （3, 3） method. The results indicated that the reliability test for quantifying EBB was passed through and the measured value was valid. The analytes showed the significant EBB （P〈0.05）. The correlation coefficient was 0.998 4 （n=5） between the amount of cyclosporine A （0.035-0.56 g·kg^-1） and the increased number of red blood cell. The relative standard deviation （RSD） on the amount of EBB was estimated to be 6.15% （n=6） by six replicated tests, and the confidence limit rate was 26.68% （n=6）. Five Danggui samples, which were collected from different cultivation areas with various morphological characters, showed the variety of EBB in the range of 21.95-44.16 U·g^-1. It is concluded that the developed method is accurate to quantify the EBB of Danggui raw materials, and is therefore suitable to assess its quality.

关 键 词：当归 补血活性 定量 质量评价 生物检定 

分 类 号：R284.1[医药卫生—中药学]