血竭含药血清对雪旺氏细胞NGF,BDNF,CNTF,LNGFR,TrkA,GDNF,GAP-43和NF-H表达的影响  

Effect of Draconis Sanguis-containing serum on NGF,BDNF,CNTF,LNGFR,TrkA,GDNF,GAP-43 and NF-H expressions in Schwann cells

作　　者：古今[1] 何新荣[1] 韩亚亮[1] 

机构地区：[1]中国人民解放军总医院药品保障中心中药房,北京100853

出　　处：《中国中药杂志》2015年第7期1392-1395,共4页China Journal of Chinese Materia Medica

摘　　要：目的:观察血竭含药血清对雪旺氏细胞NGF,BDNF,CNTF,LNGFR,TrkA,GDNF,GAP-43和NF-H表达的影响,探讨其促进周围神经再生的可能机制。方法:SD大鼠随机分为血竭给药组(灌服血竭的香油溶液)和空白对照组(等体积香油),制备血竭含药血清和空白对照血清。选取出生3 d SD大鼠双侧坐骨神经制备雪旺氏细胞;将培养的雪旺氏细胞分为血竭组和空白对照组,分别用10%血竭含药血清和空白对照血清干预培养。48 h后应用RT-PCR法检测各组雪旺氏细胞NGF,BDNF等基因mRNA表达水平。结果:镜下观察到大部分雪旺氏细胞呈双极梭形,细胞呈肩对肩或端对端排列,免疫细胞化学鉴定均呈阳性;与空白组比较,血竭组NGF,LNGFR,GDNF和GAP-43 mRNA表达上调(P<0.01),BDNF mRNA表达下调(P<0.05),CNTF和TrkA的mRNA均下调(P<0.01),NF-H mRNA无显著差异。结论:中药血竭可能是通过上调NGF,LNGFR,GDNF,GAP-43的mRNA的表达,同时下调BDNF,CNTF,TrkA mRNA来发挥其促进神经修复作用。Objective： To observe the effect of Draconis Sanguis-containing serum on the expressions of NGF, BDNF, CNTF, LNGFR, TrkA, GDNF, GAP-43 and NF-H in Schwann cells, and investigate the possible mechanism of Draconis Sanguis to promote peripheral nerve regeneration. Method： SD rats were randomly divided into 2 groups：the Draconis Sanguis group （orally administered with Draconis Sanguis-containing balm solution） and the blank group （equivoluminal balm） to prepare Draconis Sanguis-containing serum and blank control serum. Schwann cells were extracted from double sciatic nerves of three-day-old SD rats, divided into 2 groups：the Draconis Sanguis group and the blank control group, and respectively cultured with 10% Draconis Sanguis-containing serum or blank control serum. The mRNA expressions of NGF, BDNF, CNTF and other genes in Schwann cells were measured by RT-PCR analysis 48 hours later. Result： Most of the Schwann cells were bipolar spindle and arranged shoulder to shoulder or end to end under the microscope and identified to be positive with the immunocytochemical method. To compare with the blank group, mRNA expressions of NGF, LNGFR, GDNF and GAP-43 significantly increased （P〈0.01）. Whereas that of BDNF decreased significantly （P〈0.05）, and so did that of TrkA, CNTF （P〈0.01）, with no remarkable difference in NF-H-mRNA. Conclusion： Traditional Chinese medicine Draconis Sanguis may show effect in nerve regeneration by up-regulating mRNA expressions of NGF, LNGFR, GDNF and GAP-43 and down-regulating mRNA expressions of TrkA, BDNF and CNTF.

关 键 词：血竭 雪旺氏细胞 NGF BDNF CNTF 

分 类 号：R285[医药卫生—中药学]