重金属钼离子单抗的制备及其免疫学特性研究  

Preparation and Immunology Character of Monoclonal Antibody of Heavy Metal Ion Molybdenum

作　　者：范国英[1] 王自良[1] 姜金庆[1] 黄华国[1] 张海棠[1] 丁菡[1] 李艺[1] 

机构地区：[1]河南科技学院动物科学学院,河南新乡453003

出　　处：《西北农业学报》2015年第4期1-5,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金："十二五"国家科技支撑计划(2011BAK10B01);河南省高校科技创新人才支持计划(2010HASTT026)

摘　　要：为了检测水质、土壤、食品中的重金属钼离子的残留,故制备用于钼离子检测用的单克隆抗体。采用已合成的钼离子人工抗原Mo6+-ITCBE-BSA免疫Balb/C小鼠,用间接ELISA和间接竞争ELISA法筛选出备用小鼠,用细胞融合技术筛选制作单克隆抗体。结果表明:筛选出4株特异性强、敏感度高的杂交瘤细胞,其中4B3杂交瘤细胞株间接ELISA检测培养上清抗体效价为1∶(3.6×102);体内诱生法制备腹水效价为1∶(5.12×105);杂交瘤核型鉴定结果显示4B3杂交瘤细胞株染色体数目平均为99.4。4B3杂交瘤细胞株用间接竞争ELISA结果显示,IC50值为23.4μg/L,与Cr3+-EDTA的交叉反应率为81.8%,与其他金属螯合物无交叉反应。说明Mo6+-EDTA-mAb可用于Mo6+污染检测的免疫学试验。To detect residual of molybdenum ion in water quality,soil and foodstuff,the monoclonal antibody was been prepared.The experimental mouse was selected from the composited Mo6＋-ITCBEBSA to immunize Balb/C mouse by the means of indirect ELISA and competitive ELISA,and monoclonal antibody was prepared by cell fusion technology.The experimental results showed：four hybridoma cell lines characterized as powerful specificity and high sensitivity were obtained.The indirect ELISA titer in antiserum of 4B3 was 1∶（3.6×102）in supernatant of cultivation,and 1∶（5.12×105）in ascites fluid.Caryotyping indicated that the average chromosome number of hybridoma cell 4B3was99.4.The Mo-EDTA-mAb of 4B3 showed a good sensitivity with an IC50 of 23.4μg/L to Mo6＋-EDTA and 81.8% CR to Cr3＋-EDTA and little or no CR to other compounds by competitive ELISA.The prepared monoclonal antibody of Mo6＋-EDTA can be used to detect of pollution of molybdenumion with immunology tests.

关 键 词：钼离子 单克隆抗体 细胞融合技术 

分 类 号：Q813.2[生物学—生物工程]