磁纳米颗粒固定化多能硫碱弧菌(T.versutus D301)生物脱硫  

Biodesulfurization of Immobilized Sulfur Oxidation Bacteria Thioalkalivibrio versutus D301 with Fe_3O_4 Magnetic Nanoparticles

作　　者：许晓卉[1,2] 邱晓玲[3] 穆廷桢 周杰民[1,2] 白冰[1,2] 宋子煜[1] 吴丹[1] 官月平[3] 邢建民[1] 

机构地区：[1]中国科学院过程工程研究所生化工程国家重点实验室,北京100190 [2]中国科学院大学,北京100049 [3]北京科技大学材料科学与工程学院,北京100083

出　　处：《过程工程学报》2015年第2期301-306,共6页The Chinese Journal of Process Engineering

基　　金：国家高技术研究发展计划(863)基金资助项目(编号:2011AA060904);国家自然科学基金面上基金资助项目(编号:31370078)

摘　　要：采用共沉淀法合成油酸修饰的Fe3O4纳米颗粒,用其固定化Thioalkalivibrio versutus D301细胞,考察了盐碱环境下纳米颗粒固定化细胞的工艺条件,比较了固定化细胞和游离细胞的硫氧化活性,研究了固定化细胞的重复利用性能.结果表明,最佳固定化条件为Na+浓度0.6 mol/L,p H值9.5,固定化温度20℃,吸附时间10 min.固定化细胞硫氧化速率是游离细胞的81%.固定化细胞具有很好的硫氧化活性,可重复使用至少6次.Magnetic Fe304 nanoparticles were synthesized by a coprecipitation method followed by modification with oleic acid, which was used for immobilizing Thioalkalivibrio versutus D301 cells. The process parameters of cell immobilization under halo-alkaline condition, the sulfur oxidation rate of free cells and immobilized cells and recycling ability of immobilized cells were investigated. The results indicated that the optimum immobilization conditions were that the cells were immobilized for 10 rain at the conditions of 0.6 mol/L Na＋, pH 9.5 and 20 ℃. The sulfur oxidation rate of immobilized cells was 81% of free cells after activation. The coated cells not only had good sulfur oxidation activity, but also could be reused six batch cycles.

关 键 词：嗜盐嗜碱性硫氧化菌 生物脱硫 细胞固定化 油酸修饰Fe3O4纳米颗粒 

分 类 号：Q814.2[生物学—生物工程]