原儿茶酸对帕金森病模型小鼠中脑和纹状体D2DR、iNOS和TH表达的影响  

Effects of protocatechuic acid on expression of D2DR, iNOS, and TH in striatum and midbrain of Parkinson's disease model mice

作　　者：尹雪[1] 苏新云 王秀华[1] 苏佳丽[1] 廉艳 张秀丽[1] 

机构地区：[1]滨州医学院药学院,山东烟台264003

出　　处：《中草药》2015年第6期866-870,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81102828;81273037);山东省自然科学基金项目(ZR2011HM011;J11LF-86);山东省高等学校科技计划项目(J11LF-86)

摘　　要：目的考察原儿茶酸对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)致帕金森病(PD)小鼠中脑和纹状体多巴胺D2受体(D2DR)、诱导型一氧化氮合酶(i NOS)和酪氨酸羟化酶(TH)表达的影响。方法选用C57BL褐鼠为实验动物,连续7 d ip MPTP(25 mg/kg)制备PD动物模型。造模前后分别ip给予原儿茶酸(10 mg/kg)或阳性对照药美多芭(125 mg/kg)进行预防和治疗。应用Western blotting的方法,观察原儿茶酸对PD模型小鼠中脑和纹状体内D2DR、i NOS和TH表达的影响。结果 PD模型小鼠脑组织内i NOS表达升高,D2DR和TH表达下降,而原儿茶酸能够显著降低PD模型小鼠脑中i NOS的表达,提高D2DR和TH的表达量。结论原儿茶酸对MPTP诱导的PD模型小鼠有保护作用,可能通过提高PD模型小鼠中脑和纹状体内D2DR和TH的表达,降低i NOS的表达发挥作用。Objective The aim of this paper is to investigate the effects ofprotocatechuic acid （PCA） on the expression ofD2DIL iNOS, and TH in striatum and midbrain of Parkinson＇s disease （PD） mice induced by MPTP. Methods C57BL brown mice were used as experimental animals and were injected with MPTP （25 mg/kg.d） for 7 d to build PD model mice. PCA （10 mg/kg.d） and Madopar （125 mg/kg.d） had been ip administered to the PD mice for the precaution and treatment at the first and lasted for 7 d. The expression of D2DR, iNOS, and TH in the striatum and midbrain of PD mice induced by MPTP was detected by Westem blotting method. Results The results showed that iNOS expression increased while D2DR and TH expression decreased in the striatum and midbrain of MPTP-induced PD mice. However, PCA significantly decreased the expression of iNOS while obviously increased the expression of D2DR and TH in the striatum and midbrain ofMPTP-induced PD mice. Conclusion PCA has a neuroproteetive effect on the PD mice induced by MPTP, and its protective effects on mice brain are achieved at least partly by increasing TH and D2DR expression while decreasing iNOS expression.

关 键 词：帕金森病 原儿茶酸 1-甲基-4-苯基-1 2 3 6-四氢吡啶 多巴胺D2受体 诱导型一氧化氮合酶 酪氨酸羟化酶 

分 类 号：R285.5[医药卫生—中药学]