银杏叶的指纹图谱建立及质量评价研究  

Establishment of fingerprint of Ginkgo biloba leaves and study on its quality evaluation

作　　者：丁银花[1,2] 孙永成[3,2] 王振中[3,2] 毕宇安[3,2] 万琴[3,2] 徐连明[3,2] 萧伟[3,2] 

机构地区：[1]北京中医药大学,北京100020 [2]中药制药过程新技术国家重点实验室,江苏连云港222001 [3]江苏康缘药业股份有限公司,江苏连云港222001

出　　处：《中草药》2015年第6期901-905,共5页Chinese Traditional and Herbal Drugs

基　　金：江苏省科技成果转化项目(BA2012020);创新中药中试放大研究技术平台

摘　　要：目的比较不同产地、树龄、采收期银杏Ginkgo biloba叶的质量,固定欣脉胶囊处方中银杏叶的来源产地。方法建立银杏叶的HPLC指纹图谱测定方法,对不同产地、树龄、采收期银杏叶的指纹图谱进行测定,并测定不同产地、树龄、采收期银杏叶总黄酮醇苷和总萜内酯的量,对各批次银杏叶主要成分的量及指纹图谱进行对比分析。结果不同产地、树龄、采收期的银杏叶总黄酮醇苷、萜类内酯量以及指纹图谱均不同。结论所建立的指纹图谱测定方法为银杏叶的合理采收及质量评价提供了科学依据;江苏邳州、2~3年树龄、6~8月份采集的银杏叶质量较佳,可以作为欣脉胶囊处方中银杏叶的固定来源产地。Objective To compare the quality Ginkgo biloba L. leaves collected form various habitats, different tree ages, and different harvesting times and to confirm the source of G. biloba leave used in Xin Mai Capsule. Methods To establish the HPLC fingerprint of G. biloba leaves to determine the fingerprint and content of total fiavonol glucoside and terpene lactones in G. biloba leaves collected from various habitats, different tree ages, and different harvesting time, and then to analyze the results. Results The contents of total flavonol glucoside and terpene lactones and fingerprint similarity in G biloba leaves from various regions, different tree ages, and collecting times were different. Conclusion The fingerprint control method has provided the scientific basis for the reasonable collection and quality evaluation of G. biloba leaves. The G. biloba leaves in June to August, 2--3 year-old from Pizhou, Jiangsu province are better, which could be considered as the source of G. biloba leaves used in Xin Mai Capsule.

关 键 词：银杏叶 高效液相色谱法 总黄酮醇苷 总萜内酯 定量测定 指纹图谱 

分 类 号：R286.6[医药卫生—中药学]